氧化槐定碱对大鼠网状结构脑电活动及其功率谱的影响

Effect of oxysophoridine on electric activities and its power spectrum of reticulum formation in rats

目的:观察氧化槐定碱(oxysophoridine,OSR)对麻醉大鼠中脑网状结构脑生物电(EEG)变化及功率谱的影响。方法:利用脑立体定位技术,将电极置入大鼠中脑网状结构,采用单极引导法及计算机辅助FFT技术以脑电图、功率谱、频率分配为指标记录并分析OSR对大鼠中脑网状结构脑生物电变化的影响。结果:每只侧脑室注射(icv)OSR2.5,5,10mg,大鼠中脑网状结构出现以低幅慢波为主并伴有梭形睡眠波;脑电总功率下降(P<0.05),θ,α波占总频率比例升高(P<0.05)。结论:OSR具有中枢抑制作用,其作用与降低大鼠中脑网状结构电活动有关;中脑网状结构可能是OSR中枢抑制作用传导环路上的结构之一。

Objective： To observe the effect of oxysophoridine （OSR） on the EEG and its power spectrum of reticulum formation in mesencephalon of anaesthetized rat. Method： Utilizing the technique of brain stereotactic apparatus, electrodes were implanted into reticulum formation of mesencephalon. Monopolar lead and computerized FFT technique were employed to record and analyse the index of EEG, power spectrum and frequency distribution in order to study the effect of oxysophoridine on the bioelectricity change of mesencephalon reticulum formation in rats. Result： After administrating（icv） with oxysophoridine at the dose of 2. 5,5,10 mg/rat, the EEG of mesencephalon reticulum formation mainly characterized with low amplitude and slow waves accompanied by spindle-formed sleeping waves with a significant decrease of total power of EEG （ P 〈 0. 05 ） while the ratio of 0, ct waves increased in total frequency of rats（ P 〈0. 05 ）. Conclusion： Oxysophoridine possesses central inhibitory effects and its inhibitory mechanism may associate with the reduction of bioeleetricity in mesencephalon reticulum formation. Mesencephalon reticulum formation may serve as one part of the structure serving as the circuit conducting the central inhibitory effect of oxysophoridine.