2种海藻溴酚化合物的抗肿瘤作用及其机制研究

Antitumor effects and the mechanism of two kinds of bromophenols from marine algae

目的:探讨2种海藻溴酚化合物对3株肿瘤细胞Hela,MGC,BGC-823的体外抗肿瘤作用及其作用机制。方法:采用MTT比色法检测不同浓度溴酚化合物对肿瘤细胞的抑制作用;用流式细胞术分析2种化合物对3种肿瘤细胞的细胞周期和非整倍体的诱导作用。结果:2种海藻溴酚化合物对肿瘤细胞均有较强的抑制作用,其中对Hela细胞的抑制活性最强。2种海藻溴酚化合物均不能引起细胞凋亡,但能使肿瘤细胞的倍性改变,产生非整倍体,并使细胞周期受到影响。对Hela细胞和MGC细胞,低浓度能引起亚倍性的产生,较高浓度则引起G1期抑制。对BGC-823细胞,不同浓度处理均能引起G1期抑制,其抑制程度具有剂量效应。结论:海藻溴酚化合物对肿瘤细胞具有不同的生长抑制活性,其作用机制与诱导产生非整倍体和细胞周期抑制有关。

Objective： To investigate the antitumor effects of two kinds of bromophenols isolated from marine algae Rhodomela confervoides on three tumor cells of Hela, MGC and BGC-823 and their antitumor mechanism invitro. Method： MTT method was employed to assay the inhibitory effects of marine bromphenols with various concentrations. Flow cytometry （FCM） was used to study the cell cycle and aneuploid induction. Result： Both of two bromophenols showed cytotoxie activities on the tested tumor cells. Hela cells were proved to be the most sensitive to the marine bromophenols. Although they cotddn＇t cause apoptosis of the tumor cells, the aneuploid and cell cycle inhibition were detected. For Hela and MGC cells, hypoploid was observed under low drug concentrations, while G1 phase block was caused by higher drug concentrations. For BGC-823 cells, G1 phase inhibition was observed for different drag concentrations, and the inhibitiory effect showed dose-dependent. Conclusion： Marine bromophenol can inhibit the proliferation of three tumor ceils, and the mechanism was probably aneuploid induction and cell cycle inhibition.