摘要
目的:建立LC-MS测定大鼠血浆中芍药苷含量的方法,并探讨其在大鼠体内的药动学行为。方法:大鼠灌胃不同剂量的炒白芍提取物后不同时间点采血,LC-MS法测定血药浓度,拟合药动学模型并计算相关药动学参数。结果:芍药苷在2.5~500μg·L-1(r=9994)线性关系良好。方法学考察均符合要求。大鼠灌胃炒白芍提取物0.2,0.4,0.8g·kg-1后,半衰期基本一致,AUC与给药剂量之间呈现良好的线性关系,属于线性动力学过程。结论:该法专属性强,灵敏度高,可用于芍药苷的体内定量分析,芍药苷在大鼠体内动力学过程符合一级吸收二室模型。
Objective: To develop a LC-MS method to determine paeoniflorin concentration in rats plasma. The method was applied to investigate pharmacokinetics of paeoniflorin in rats in vivo. Method: Blood samples were collected at different time after oral administration of Radix Paeoniae Alba extract at doses of O. 2, 0. 4, 0. 8 g · kg^-1. The paeoniflorin concentration in plasma was determined by LC-MS method. Pharmacokinetic parameters were fitted by WinNonlin 5.1 software package. Result: The linear range and the average recovery of paeoniflorin were 2. 5-500 μg · L^-1 ( r = 999 4). and more than 80% ( n = 5 ), respectively. The inner- and inter-days precision were both less than 15%. The T1/2 was similar. The relationship between dose and AUC showed good linearity. Conclusion: The method described in this report has high sensitivity and selectivity, and was suitable for pharmacokinetic study of paeoniflorin. The kinetic process of paeoniflorin in palsma showed two-compartment model after oral administration of Radix Paeoniae Alba extract at doses of 0.2, 0. 4, 0. 8 g · kg^-1 to rats.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第9期1193-1196,共4页
China Journal of Chinese Materia Medica
基金
国家科学自然基金重点项目(30930114)
国家自然科学基金项目(90709017)
国家"十一五"科技支撑计划项目(2006BAI08B04-4)