黄瓜香提取物对大鼠肝纤维化拮抗作用

Anti-hepatic fibrosis effect of Viola diffusa Ging in rats

目的研究黄瓜香对大鼠肝纤维化拮抗作用。方法将48只大鼠随机分为正常对照组、模型组、生理盐水组和黄瓜香干预组,全自动生化分析仪检测丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、碱性磷酸酶(ALP),苏木素-伊红(HE)染色观察肝脏显微结构,免疫组织学染色检测Ⅰ、Ⅲ型胶原、α-平滑肌肌动蛋白(α-SMA)及转化生长因子β1(TGF-β1)。结果黄瓜香干预组ALT,AST和ALP分别为(70.8±12.3),(112.3±26.4)和(169.1±21.2)U/L,与模型组比较,明显降低(P<0.01);黄瓜香干预组肝Ⅰ、Ⅲ型胶原、α-SMA及TGF-β1表达与正常对照比较差异无统计学意义(P>0.05),而与模型组比较,表达则明显增强,差异均有统计学意义(P<0.01)。结论黄瓜香提取物可干预肝纤维化过程,明显减少纤维化组织面积。

Objective To study antagonisic effect of Vioal diffusa Gings（VDG） on hepatic fibrosis in rats.Methods Forty-eight rats were randomly divided into four groups：normal control group,model control group,normal sodium group and VDG treated group.The contents of alanine aminotransferase（ALT）,aspartate aminotransferase（AST） and alkaline phosphatase（ALP） were measured with full automatic biochemical analyzer.Hepatic microstructure was showed by HE staining.The expression of collagen alphal Ⅰ,Ⅲ,α-smooth muscle actin（α-SMA） and transforming growth factor-β1（TGF-β1） were detected with immunohistochemistry dyeing.Results Compared with the model group,the contents of ALT,AST and ALP of VDG treated group remarkably decreased（P0.01）.In VDG treated group,the hepatic lobe structure was normal and the slender textile fiber gap was minor with few inflammatory cell infiltration.There was a significant difference in the average grey degree of collagen alphal Ⅰ,Ⅲ,α-SMA and TGF-β1 ibetween normal control,model control and VDG treated group（P 0.01）.Conclusion The alcohol extract from VDG showed protective effect against liver fibrosis and could alleviate the degree of degeneration and necrosis induced by CCl4.