大黄酸对大鼠肾小管上皮细胞肥大抑制作用

Rhein inhibits hypertrophy of renal proximal tubular epithelial cells induced by high glucose in rats

目的观察大黄酸(Rhein)对高糖培养大鼠近端肾小管上皮细胞肥大影响。方法无菌分离SD大鼠单根近球小管并培养;高糖(30 mM)培养肾小管上皮细胞,加入30,15,5 mg/L大黄酸,作用72 h,检测细胞体积、3H-亮氨酸掺入量及蛋白质含量等细胞肥大指标。结果高糖培养72 h导致细胞体积明显增大,3H-亮氨酸掺入量及细胞内蛋白质含量分别为(16 225.0±1 476.1)cpm/105细胞和(4.43±0.38)μg/105细胞,明显增加;30 mg/L大黄酸处理72 h后,3H-亮氨酸掺入量及细胞内蛋白质含量分别为(10 734.0±978.9)cpm/105细胞和(4.04±0.23)μg/105细胞,大黄酸可明显抑剂高糖所致细胞体积增大、3H-亮氨酸掺入量及细胞内蛋白质含量的升高。结论大黄酸可抑制高糖诱导的肾小管上皮细胞肥大。

Objective To investigate the effect of rhein on the hypertrophy of renal proximal tubular epithelial cells induced by high glucose in rats.Methods Studies were performed on anesthetized SD rats.Renal proximal tubular epithelial cells were gained by microdissection and were cultured in RPMI 1640 medium.The cell types were idenfied with immunocytochemistry.The cells were incubated with high glucose（30 mM） to induce cell hypertrophy.To observe the effcet of rhein on the hypertrophy induced by high guucose,the cells were cultured with different concentrations of rhein（30,15 and 5 mg/L） for 72 hr.Then the cell size,3H-lecuine incorporation,and cellular protein content were detected to observe the changes.Results High glucose（30 mM） resulted in enlargment of renal proximal tubular epithelial cells,increases of 3H-leucine incorpiration and cellular protein content significantly.On the contrary,rhein inhibited the hypertrophy of renal proximal tubular epithelial cells induced by high glucose.Rhein（30 mg/L） decreased cell size,3H-leucine incorporation and cellular protein content significantly.Rhein（15 mg/L） decreased 3H-leucine incorporation and cellular protein content.Rhein（5 mg/L） decreased cellular protein content.Conclusion Rhein can inhibit the hypertrophy of renal proximal tubular epithelial cells induced by high glucose in rats.