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鸡致病性大肠杆菌O_(78)菌株iss基因的克隆与测序

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摘要 对1株O78血清型鸡源致病性大肠杆菌采用PCR方法检测iss基因的存在。结果显示,鸡E.coliO78菌株携带iss基因,但采用目前通用的PCR方法并不能检测出iss基因,必须经套式PCR扩增才能检测出来。O78菌株所携带iss基因的序列与已知禽大肠杆菌iss基因序列同源性为99.4%,与人源大肠杆菌iss基因同源性为90.3%,与λ噬菌体bor基因序列同源性为87.7%,显示了该基因的保守性。
作者 樊琛 王宇 刘桂芹 王亚君 李一经
机构地区 山东省东北农业大学动物医学院 聊城大学农学院 山东省聊城市人民医院呼吸内科 东北林业大学野生动物学院
出处 《江苏农业科学》 CSCD 北大核心 2010年第2期40-42,共3页 Jiangsu Agricultural Sciences
关键词 鸡大肠杆菌 ISS基因 毒力 套式PCR 同源性
分类号 S858.31 [农业科学—临床兽医学]
  • 相关文献

参考文献14

  • 1Janben T,Schwarz C,Preikschat P,et al.Virulence-associated genes in avian pathogenic Escherichia coli(APEC) isolated from internal organs of poultry having died from colibacillosis[J].Int J Med Microbiol,2001,291(5):371-378.
  • 2Johnson T J,Giddings C W,Horne S M,et al.Location of increased serum survival gene and selected virulence traits on a conjugative R plasmid in an avian Escherichia coli isolate[J].Avian Dis,2002,46(2):342-352.
  • 3Nolan L K,Giddings C W,Horne S M,et al.Complement resistance,as determined by viable count and flow cytometric methods,and its association with the presence of iss and the virulence of avian Escherichia coli[J].Avian Dis,2002,46(2):386-392.
  • 4Chuba P J,Leon M A,Banerjee A,et al.Cloning and DNA sequence of plasmid determinant iss,coding for increased serum survival and surface exclusion,which has homology with lambda DNA[J].Mol Gen Genet,1989,216(2/3):287-292.
  • 5Dho-Moulin M,Fairbrother M.Avian pathogenic Escherichia coli(APEC)[J].J Vet Res,1999,30(2/3):299-316.
  • 6Johnson J R,Sannes M R,Croy C,et al.Antimicrobial drug-resistant Escherichia coli from humans and poultry products,minnesota and Wisconsin,2002-2004[J].Emerg Infect Dis,2007,13(6):838-846.
  • 7Fantinatti F,Silveira W D,Castro A F.Characteristics associated with pathogenicity of avian septicaemic Escherichia coli strains[J].Vet Microbiol,1994,41(1/2):75-86.
  • 8Ramirez Santoyo R M,Moreno Sala A,Almanza Marquez Y.Avian Escherichia coli virulence factors associated with coli septicemia in broiler chickens[J].Rev Argent Microbiol,2001,33(1):52-57.
  • 9Wooley R E,Spears K R,Brown J,et al.Relationship of complement resistance and selected virulence factors in pathogenic avian Escherichia coli[J].Avian Dis,1992,36(3):679-684.
  • 10Nolan L K,Wooley R E,Brown J,et al.Comparison of a complement resistance test,a chicken embryo lethality test,and the chicken lethality test for determining virulence of avian Escherichia coli[J].Avian Dis,1992,36(2):395-397.

二级参考文献31

  • 1王亚君,樊琛,李一经.fimC基因与鸡源大肠杆菌致病性的相关[J].畜牧兽医学报,2004,35(6):680-684. 被引量：16
  • 2Janben T, Schwarz C, Preikschat P, et al. Virulenceassociated genes in avian pathogenic Escherichia coli (APEC) isolated from internal organs of poultry having died from colibacillosis [J]. Int J Med Microbiol, 2001, 291(5):371-378.
  • 3Dho-Moulin M, Fairbrother J M. Avian pathogenic Escherichia coli(APEC)[J]. J Vet Res, 1999, 30(2-3) :299-316.
  • 4Fantinatti F, Silveira W D, Castro A F. Characteristics associated with pathogenicity of avian septicaemic Escherichia coli strains[J]. Vet Microbiol, 1994, 41(1-2) :75-86.
  • 5Ramirez Samoyo R M, Moreno Sala A, Almanza Marquez Y. Avian Escherichia coli virulence factors associated with coil septicemia in broiler chickens[J]. Rev Argent Microbiol, 2001, 33(1):52-57.
  • 6Wooley R E, Spears K R, Brown J, et al. Relationship of complement resistance and selected virulence factors in pathogenic avian Escherichia coli[J]. Avian Dis, 1992, 36(3):679-684.
  • 7Nolan L K,Wooley R E,Brown J, et al. Comparison of a complement resistance test,a chicken embryo lethality test, and the chicken lethality test for determining virulence of avian Escherichia coli [J]. Avian Dis, 1992,36(2):395-397.
  • 8Nolan, L K, Wooley R E,Cooper R K. Transponson mutagenesis used to study the role of complement resistance in the virulence of an avian E. coli isolate[J]. Avian Dis, 1992, 36(2):398-402.
  • 9Johnson T J, Giddings C W, Horne S M, et al. Location of increased serum survival gene and selected virulence traits on a conjugative R plasmid in an avian Escherichiacoli isolate[J]. Avian Dis, 2002, 46(2): 342-352.
  • 10Nolan L K, Giddings C W, Horne S M, et al. Complement resistance, as determined by viable count and flow cytometric methods, and its association with the presence of iss and the virulence of avian Escherichia coli[J]. Avian Dis, 2002, 46(2): 386-392.

共引文献21

江苏农业科学
2010年 第2期

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