摘要
目的探讨骨髓增生异常综合征(MDS)患者CD4+ CD25+调节性T细胞(CD4+ CD25+ Treg细胞)和调控基因FOXP3的表达及意义。方法分离22例MDS患者[MDS组,根据MDS分型标准分为:难治性贫血组(RA组)6例、难治性血细胞减少伴有多系发育异常组(RCMD组)11例、难治性贫血伴原始细胞增多组(RAEB组)5例]和8例正常健康者骨髓单个核细胞,采用免疫荧光标记和流式细胞仪检测CD4+ CD25+ Treg细胞占CD4+ T细胞比例;提取骨髓单个核细胞RNA,采用RT-PCR检测FOXP3基因的表达水平。结果MDS组CD4+ CD25+ Treg细胞比例、FOXP3基因表达水平与正常对照组比较,差异均无统计学意义(均P>0.05);RA组、RAEB组CD4+ CD25+ Treg细胞比例、FOXP3基因表达水平与正常对照组比较,差异均有统计学意义(均P<0.01);RCMD组CD4+ CD25+ Treg细胞比例、FOXP3基因表达水平与正常对照组比较,差异均无统计学意义(均P>0.05);RCMD组、RAEB组CD4+ CD25+ Treg细胞比例、FOXP3基因表达水平与RA组比较,差异均有统计学意义(均P<0.01);RA组、RCMD组CD4+ CD25+ Treg比例、FOXP3基因表达水平与RAEB组比较,差异均有统计学意义(均P<0.01)。结论MDS患者CD4+ CD25+ Treg占CD4+ T细胞比例及FOXP3基因表达水平随预后危险级别的升高而升高,提示免疫异常是MDS疾病的发生、发展的一个促进因素。
Objective To explore the expression levels and the clinic significance of CD4^+CD25^+ regulatory T cell (CD4^+ CD25^+ Treg cell) and regulator and control gene FOXP3 in the myelodysplastic syndrome (MDS) patients.Methods The expression levels of CD4^+ CD25^+ Treg was detected by means of immunofluorescence and flow cytometry in 24 patients with MDS [MDS group:divide by the standard of MDS grouping:refractory anemia group (RA group) 6 examples,refractory cytopenia with multilineage dysplasia group (RCMD group) 11 examples,refractory anemia with excess blasts group(RAEB group) 5 examples] and 8 normal controls,respectively,that of FOXP3 by real-time quantitative RT-PCR.Results The expression levels of CD4^+ CD25^+ Treg and FOXP3 in the MDS group were not obviously different with the normal control group(all P〈0.05);patients with RA group and RAEB group,The expression levels of CD4^+ CD25^+ Treg and FOXP3 were obviously different with the normal control group (all P〈0.01);The expression levels of CD4^+ CD25^+ Treg and FOXP3 in the RCMD group were not obviously different with the normal control group (all P〈0.05);patients with RCMD group and RAEB group,The expression levels of CD4^+ CD25^+ Treg and FOXP3 were obviously different with the RA group(all P〈0.01);patients with RA group and RCMD group,The expression levels of CD4^+ CD25^+ Treg and FOXP3 were obviously different with the RAEB group(all P〈0.01).Conclusion The percentage of CD4^+ CD25^+ Treg in CD4^+T cell and the expression level of FOXP3 depends on the risk category of MDS;higher risk indicates more percentages and expressions,suggesting that immunologic abnormality is a promoting factor in the onset and progression.
出处
《实用临床医学(江西)》
CAS
2010年第1期5-7,10,共4页
Practical Clinical Medicine