DNS法测定还原糖含量时最适波长的确定

Optimal Wavelength for Determining the Content of Reducing Sugar by DNS Method

探讨DNS法测定还原糖含量时的最适波长。以DNS为显色剂，用分光光度法分析测定了木糖和葡萄糖显色液的最大吸收波长和最适测定波长。结果表明，木糖和葡萄糖与DNS显色剂反应后，均在490nm处有最大吸收峰。标准曲线糖含量范围为0～40mg／L时，OD值在520nm处的群最接近于1，精确度和回收率均较高。提示DNS法测定还原糖含量时，520nm为最适测定波长。

In this study, the OD values of xylose and glucose under different wavelength were determined. The results showed that the DNS reaction solution of xylose and glucose have the same maximum absorption wavelength （490nm） and the same optimal determining wavelength （520nm）. When determine the content of xylose and glucose under the wavelength of 520nm, the precision and recollection rate are comparatively high and the line range of reducing sugar is 0-40mg/L.