一种新的皮肤黑色素细胞及肥大细胞缺乏的小鼠模型

A novel mouse model with deficiency of melanophore and mast cells in the skin

目的分析Kit基因突变的W-4Bao白斑小鼠的表型特征。方法遗传试验观察突变小鼠的大体表型,多巴染色法观察黑色素细胞,甲苯胺蓝染色法观察肥大细胞。结果突变基因杂合子小鼠腹部三角形白斑,肢端、尾端白化;皮肤组织内黑色素细胞及肥大细胞无明显差异。突变纯合子小鼠全身被毛白色,眼周、耳廓、背部局部被毛末端灰黑色,黑眼;皮肤组织内多巴阳性黑色素细胞、肥大细胞较对照组明显减少几近完全缺失。结论 Kit基因错义突变不仅导致了W-4Bao杂合子及纯合子小鼠黑色素细胞缺乏,同时影响了肥大细胞的发育。

Objective To analyze the phenotypes of W-4Bao mouse with Kit gene mutation. Methods Genetic testing was used to observe the phenotypes of the mutative mice and histopathological examinations were used for analysis of melanophore and mast cells. Results The heterozygote （W-4Bao/＋） mouse has the features of white belly, white limb terminals and white tail tip. There was no significant difference of the number of Dopa（ ＋ ） melanophore and mast cells in the skin between the W-4Bao＋ and wild type mouse. The homozygote （W-4Bao/4Bao） mouse has the phenotype of black eyes and albino body, except light black hair in limited area of the back and around eyes and auricles. There were nearly no Dopa （ ＋ ） melanophore and mast cells in the skin of the W-4Bao/4Bao mouse compared with the wild type one. Conclusion The missense mutation iof Kit gene leads to deficiency of melanophore and simultaneously influences the development of mast cells in the skin of the W-W-4Bao mouse and the W-4Bao/4Bao mouse.