散发性结直肠癌FHIT基因杂合性缺失与临床病理特征的关系

The Relationship between Loss of Heterozygosity of FHIT Gene and Clinical Pathological Characteristics in Sporadic Colorectal Cancer

目的探讨散发性结直肠癌FHIT基因杂合性缺失与临床病理特征的关系。方法收集散发性结直肠癌新鲜标本及其配对的癌旁正常组织标本63例,选取微卫星位点D3S1300和D3S1234标记FHIT基因的杂合性缺失,采用PCR-单链构象多态性(SSCP)-银染技术检测以上微卫星位点,并结合患者的临床资料(年龄、性别、肿瘤位置、肿瘤分化程度、Dukes分期及有无淋巴结转移)分析二者之间的关系。结果 FHIT基因杂合性缺失的检出率为22.0%,其与肿瘤的分化程度、有无淋巴结转移显著相关(p<0.05),与其他临床病理参数无明显关系(p>0.05)。结论 FHIT基因的杂合性缺失可能在结直肠癌的发生发展中起着重要作用。

Objective To study the relationship between loss of heterozygosity （LOH） of FHIT gene and clinical pathological paracteristics in sporadic colorectal cancer. Methods To collect 63 cases of fresh sporadic colorectal cancer tissues and matched normal tissues. LOH of FHIT gene were detected at two microsaterllite locis D3S1300 and D3S1234 by the method of polymerase chain reaction single strand conformation polymorphism （PCR-SSCP） and silver staining. LOH of FHIT gene were analyzed in combination with the zlinical data （age, sex, etc）. Results The rate of LOH of FHIT gene was 22.0%. LOH of FHIT gene had a significant correlation with tumor ：lifferentiation and lymph node metastasis （p 〈0.05）, while no significant correlation with other clinical pathological characteristics （p 〉0.05）. Conclusion LOH of FHIT gene maybe playe an important role in the occurrence and development of sporadic colorectal cancer.