黄竹清脑颗粒对痰热腑实证脑出血大鼠血浆CCK的影响

Influence of "Huangzhu Qingnao Granule" on plasma cholecystokinin in rats with cerebral hemorrhage of phlegm heat in bowels

目的探讨黄竹清脑颗粒对痰热腑实证脑出血大鼠模型血浆中胆囊收缩素(CCK)的影响。方法SD大鼠60只,随机分为黄竹清脑颗粒大、中、小剂量组、清开灵颗粒组、痰热腑实证模型组和空白对照组,每组10只。采用脑内注射胶原酶Ⅶ-肝素混合液配合大鼠自体粪便灌胃的方法建立痰热腑实证脑出血大鼠模型,观察各组大鼠症状和神经体征用药后的变化,并用放免法测定大鼠血浆中CCK的变化。结果黄竹清脑颗粒可明显减轻痰热腑实证脑出血大鼠粪便干结、烦躁、鼻分泌物多、喉中痰鸣等痰热腑实证症状表现,可改善脑出血后神经体征,同时明显降低痰热腑实证脑出血大鼠血浆中CCK含量,且黄竹清脑颗粒大、中剂量组优于清开灵颗粒组(P<0.05)。结论痰热腑实证脑出血的形成与血浆CCK水平呈正相关,黄竹清脑颗粒治疗后随着痰热腑实证脑出血大鼠症状的减轻,大鼠血浆CCK含量明显降低,黄竹清脑颗粒有类似血浆CCK拮抗兴奋性氨基酸神经毒性作用,从而起到减轻颅内血肿及脑水肿、脑缺血后的再灌注损伤,进而保护脑细胞的作用。

Objective To probe into the influence of ＂Huangzhu Qingnao Granule＂ on cholecystokinin（CCK） in the rats with cerebral hemorrhage of phlegm heat in the bowels.Methods Sixty rats were randomized into six groups,10 rats in each group.Cerebral hemorrhage of phlegm heat in the bowels was duplicated by injecting collagenase Ⅶ with heparin and gastric gavage of rats＇ excrement and urine.The rats＇ symptoms and nerve signs were observed,and the content of plasma CCK was detected.Results ＂Huangzhu Qingnao Granule＂ could obviously relieve the rats＇ symptoms and nerve signs,and reduce the content of CCK;and high-dose ＂Huangzhu Qingnao Granule＂ worked better than ＂Qingkailing Granule＂（P〈0.05）.Conclusion The content of CCK has positive correlation with cerebral hemorrhage,and ＂Huangzhu Qingnao Granule＂ can relieve rats＇ symptoms and signs and reduce the content of CCK to treat cerebral hemorrhage.