摘要
采用实时荧光定量RT-PCR检测吉富罗非鱼感染链球菌后脑和脾脏中热休克蛋白70(hsp70)基因表达情况,并按2-△△CT分析方法、使用7500 Software v2.0.1进行分析。结果表明,罗非鱼感染链球菌48 h内,hsp70 mRNA在脑组织表达变化不明显,而在脾脏中表达升高,攻毒感染24 h后hsp70 mRNA表达量是正常脾脏组织的5.5倍多,说明hsp70在罗非鱼链球菌感染的免疫反应中具有重要作用。研究结果为进一步分析hsp70在罗非鱼感染链球菌过程中所行使的功能奠定基础。
The present experiment was conducted to detect and analyze the expressions of hsp70 gene in brain and spleen of tilapia infected with Streptococcus using real-time fluorescence quantitative PCR, the 2^-△△CT method and 7500 Software v2.0.1. The results showed no significant changes in the expression of hsp70 mRNA in brain after 48h infection, while the expression of hsp70 was increased in spleen significantly and was 5.5 times higher than that of the control after 24h infection, which suggested that hsp70 may play an important role in immune response of tilapia infected with Streptococcus. These results laid down the foundation for further research on the function of hsp70 in tilapia infected with StreptoCOCCUS.
出处
《广西农业科学》
CAS
CSCD
2010年第4期371-374,共4页
Guangxi Agricultural Sciences
基金
国家科技支撑项目(2008BADB9B04)
