高效液相色谱法检测克氏原螯虾组织中氟苯尼考及氟苯尼考胺

Simultaneous determination of florfenicol and florfenicol amine in tissues of Procambarus clarkii by high performance liquid chromatography

建立了克氏原螯虾（Procambarus clarkii）血淋巴、肌肉、肝胰脏中氟苯尼考及氟苯尼考胺的高效液相色谱检测方法。采用Agilent Eclipse XDB-C18（250mm×4.6 mmi.d,5μm）反相色谱柱,以A液（乙腈）：B液（0.04 mol/L NaH2PO4、0.004 mol/L十二烷基硫酸钠和0.10%三乙胺）（体积比为30∶70）为流动相,225 nm紫外检测波长完成分析。结果显示：本方法在0.025~20 mg/L范围内浓度与峰面积呈良好的线性关系;组织的加标回收率为78.8%~94.2%,相对标准偏差小于8.16%,氟苯尼考在血淋巴、肌肉、肝胰脏的检出限分别为0.01 mg/L、0.02 mg/kg、0.03 mg/kg,氟苯尼考胺则为0.015 mg/L、0.03 mg/kg、0.05 mg/kg。

A HPLC method was developed for simultaneous determination of florfenicol（FF）and its metabolite florfenicol amine（FFA）in the hemolymph,muscle and hepatopancreas of crayfish.The analysis column was reversed-phase column of Agilent Eclipse XDB-C18（250 mm×4.6 mm i.d,5 μm）,the mobile phase was solution A（acetonitrile）and solution B（0.04 mol/L sodium dihydrogen phosphate,0.004 mol/L SDS and 0.10% triethylamine）（30∶70,V∶V）,UV detector was set at 225 nm.The results showed that：the standard curves were linear in the investigated ranges of 0.025-20.0 mg/L,and the recovery values of florfenicol and florfenicol amine were from 78.8% to 94.2%,relative standard deviations were less than 8.16%.The limit of detection for florfenicol and florfenicol amine were 0.01 mg/L and 0.015 mg/L in hemolymph,0.02 mg/kg and 0.03 mg/kg in muscle,0.03 mg/kg and 0.05 mg/kg in hepatopancrea respectively.