HPLC测定胆石颗粒中大黄素的含量

Determination of emodin in Danshi Granules by HPLC

目的测定胆石颗粒中大黄素的含量。方法采用高效液相色谱法,以ODS为色谱柱,甲醇-0.1%磷酸溶液(85∶15)为流动相,检测波长为254nm。结果胆石颗粒中大黄素的含量在0.40～4.00μg.mL-1线性关系良好(r=0.9993)。平均回收率97.59%,RSD=0.05%。结论本方法测定简便、快速,测定结果准确。

OBJECTIVE To determine the content of emodin in Danshi Granules. METHODS HPLC was used. the stationary phase was ODS and the mobile phase was methanol-0.1% phosphoric acid （85：15）. the UV detection wavelength was 254nm. RESULTS there was good linear relationship within the concentration range of 0.40--4.00μg·mL^-l of emodin in Danshi Granules （r = 0. 9993） . The average recovery was 97.59 %, RSD = 0.05 % .CONCLUSION This method is simple, fast and accurate.