圆柏果实水浸提液化感作用的初步研究

Preliminary Study on Allelopathic Effect of Aqueous Extract of Sabina chinensis L. Fruits

圆柏(Sabina chinensis L.)成年母树周围很少有实生苗生长,为了检测该植物组织是否具有抑制种子发芽的作用,通过测定圆柏果皮和种子水浸提液对萝卜、白菜和圆柏种子萌发及幼苗根长和苗高的影响,对圆柏果实化感物质的作用进行了研究。结果显示:在高浓度0.1 g/mL果皮水浸提液处理下,萝卜和白菜种子的萌发、苗高和根长生长均受到显著的抑制作用,随着浸提液浓度的降低,其抑制作用逐渐减弱;低浓度(0.01 g/mL,0.03 g/mL)的果皮水浸提液对萝卜种子的萌发、幼苗苗高和根长均表现为促进作用,对白菜种子萌发、幼苗苗高和根长有抑制作用;圆柏种子浸提液对萝卜、白菜种子萌发及幼苗生长的抑制作用不显著。圆柏果实水浸提液对萝卜和白菜具有明显的化感作用,化感物质主要存在于果皮中,而且圆柏果实的化感作用对参加试验的萝卜和白菜种子具有选择性。圆柏种子在该试验中未发芽,TTC法检测参加试验圆柏种子的活力,发现果皮浸提液处理的圆柏种子有34%具有生活力,子叶在转绿;种子浸提液处理的圆柏种子有37%具有生活力,子叶也在转绿,圆柏种子的发芽率比较低,很大程度上取决于种子的质量,圆柏对自身种子的发芽及幼苗生长的自毒效应需要进一步研究。

There are a few seedlings around the adult whether the plant tissue inhibit the seed germination and mother trees of Sabina chinensis L. In order to understand seedlings growth, the allelopathie effect of aqueous extract of Sabina chinensis L. fruits is researched after evaluating the bio-aetivities of the aqueous extracts from the peels and seeds of Sabina chinensis L. on the seed germination and seedling growth of radish, Chinese cabbage and Sabina chinensis L. The results show that, when the concentration of aqueous extract from peels was 0.1 g/mL, the germination ratio, seedling shoot length and root length of radish and Chinese cabbage were markedly inhibited. The allelo- pathic inhibition was decreased with the decrease of aqueous extract concentration, when the aqueous extract concentration was decreased to 0.01 g/mL or 0.03 g/mL, the germination ratio, seedling shoot length and root length of radish were promoted, however, the seed germination and seedling growth of Chinese cabbage were still inhibited The inhibition of aqueous extract from seeds to the seed germination and seedling growth of radish and Chinese cabbage was not so significant. The allelopathic effects of aqueous extracts from both peels and seeds on radish and Chinese cabbage were significant. Allelochemicals are mainly contained in the pericarp, and they were selective for the tested plants. The seeds of S. chinensis L. did not geminate in the test. TTC was used to assay the vitality of the seeds of S. chinensis L. , it was found that 34% seeds treated with the extract from peels were valid, and the cotyledons became green, 37% seeds treated with the extract from seeds were valid, and the cotyledons became green. Seed germination rate of S. chinensis L. was relatively low, which was closely related to the quality of seeds.