摘要
基于PCR介导的基因敲除原理,采用Cre-LoxP重组系统技术,对2倍体酿酒酵母的SS04菌株的gdh1基因进行敲除操作,构建gdh1基因完全缺失型的酿酒酵母重组菌SS17菌株,进而为后续重组酵母的乙醇代谢研究打下基础.
Knock out the gdhl gene in the diploid Saccharomyces cerevisiae SS04 by the Cre-LoxP recombination system which based on the principle of PCR-mediated gene knocking out. The result is that the recombinant Saccharomyces cerevisiae SS17 of gdhl gene completely deletion form is constructed. And laying a foundation for the research of ethanol metabolism in recombinant yeast.
出处
《福建师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第3期52-56,共5页
Journal of Fujian Normal University：Natural Science Edition
基金
福建省自然科学基金资助项目(2007J0325)
农业部现代农业产业技术体系建设专项资金(ngcytx-024-01-20)
农业部公益性行业(农业)科研专项项目(nyhyzx07-019)
农业部"948"项目(2006-G37)
