洋葱伯克霍尔德氏菌CAS19嗜铁素合成相关基因cepR的克隆及生物信息分析

Cloning and Bioinformatic Analysis of the Siderophore Synthesis-Related Gene CepR from Burkholderia Cepacia CAS19

本研究采用PCR方法克隆了洋葱伯克霍尔德氏菌CAS19嗜铁素合成相关基因cepR,并对cepR基因编码蛋白的结构和功能进行生物信息学分析和预测。同源性分析表明,CAS19的cepR核苷酸序列与Burkholderia cepacia LMG1222cepR基因的同源性为99%;cepR基因全长768bp,包含一个完整的231bp的开放阅读框架,编码239个氨基酸;该基因编码蛋白分子量为26.59kD,理论的等电点为5.55,含有一个LuxR型HTH结构域,在氨基酸残基的不同区域分布有酪蛋白激酶Ⅱ磷酸化位点、蛋白激酶C磷酸化位点、酪氨酸激酶磷酸化位点、N-肉豆蔻酰化位点和N-糖基化位点,还有一个明显的跨膜结构。本研究结果将为洋葱伯克霍尔德氏菌嗜铁素合成相关研究提供调控基因信息和参考依据,并为其进一步开发和利用奠定基础。

In this study, we cloned a full-length cDNA of siderophore synthesis-related gene which was named as cepR, by using PCR technology, and we also analyzed and forecasted the biological information for the structure and function of protein encoding by cepR gene. The homology analysis results indicated that the nucleotide sequence of CAS19 cepR have 99% identity with that ofBurkholderia cepacia LMG1222. The total length ofCAS19 cepR gene was 768 bp containing a 231 bp complete open reading frame that encoded 239 amino acids. The molecular mass and theoretical isoelectric point of protein which encoding by cepR gene was 26.59 kD and 5.55, and the protein containing a LuxR-type HTH structural domain, with several casein kinase Ⅱ phosphorylation site, protein kinase C phosphorylation site, Tyrosine kinase phosphorylation site, N-myristoylation site, N-glycosylation site and a transmembrane helices distributed at different domain of its amino acid residue. The results of this study should be provided information of controlling gene and reference of genetic for relative research of Burkolderia cepacia siderophore syn thesis, and also for laying the foundation for its further application and development.