肿瘤干细胞标记物CD44^+CD24^(low/-)与MMP-9在乳腺癌组织中的表达及其意义

Expression and Significance of Tumor Stem Cancer Marker CD44^+CD24^(low/-) and MMP-9 in Breast Cancer

目的研究肿瘤干细胞标记物CD44+CD24low/-以及基质金属蛋白酶-9(MMP-9)的表达与肿瘤临床病理参数之间的关系。方法采用免疫组织化学方法检测109例乳腺癌组织中的CD44、CD24及MMP-9的表达并对结果进行分析。结果(1)109例乳腺癌组织中CD44、CD24及MMP-9的表达率分别是47.7%、50.5%及40.4%,其中CD44的阳性表达率与乳腺癌病理分级密切相关(P<0.05);(2)109例乳腺癌组织中,CD44+CD24low/-肿瘤干细胞标记阳性例数为32例,随乳腺癌病理分级的升高,CD44+CD24low/-肿瘤干细胞比率也明显增加(P<0.05);(3)32例CD44+CD24low/-肿瘤干细胞标记阳性乳腺癌组织中,MMP-9蛋白的表达与淋巴结转移成负相关(P<0.05)。结论高度恶性乳腺癌组织中富于肿瘤干细胞,乳腺癌早期CD44+CD24low/-TSC中MMP-9蛋白表达增加可能对肿瘤干细胞(TSC)生长及浸润中起着一定作用。

Objective To investigate the expression of Cancer Stem Cancer Marker CD44 ＋ CD24low/- and matrix metalloproteinase-9 （MMP-9） in breast cancer and its relationship with the clinical characteristics of tumors. Methods In one hundred and nine cases of breast cancer tissues CD44,CD24 and MMP-9 were detected with immunohistochemisrty. Results （ 1 ） The expression rate of CD44, CD24 and MMP-9 protein was 47.7% ,50.5% and 40.4% in 109 cases of breast cancer respectively. The ex- pression rates of CD44 protein was related with breast cancer pathological grades （P 〈 0.05 ）. （2） Thirty two positive cases of CD44 ＋ CD24low/- were detected in 109 cases of breast cancer. The expression of CD44 ＋ CD24low/- was closely related with breast cancer pathologic grades. （3） The expression of MMP-9 protein was in inverse correlations with lymphoid metastasis in CD44 ＋ CD24low/- positive cases （P 〈 0.05 ）. Conclusion CD44 ＋ CD24low/- TSC is often be found in poor differentiation breast cancer tissues. And MMP-9 protein can be an important factor in invasion of CD44 ＋ CD24low/- TSC in early stage of breast cancer.