微吸管技术在动脉力学性质研究中的应用

APPLICATION OF MICROPIPETTE TECHNIQUE IN RESEARCH OF MECHANICAL PROPERTIES OF ARTERY

动脉力学性质的研究对于动脉正常生理特性的分析和病理状态下性状改变的研究有着重要意义。以往的研究多采用以液体或气体给动脉加压或轴向拉伸等手段，揭示了动脉轴向、周向和径向的力学特性。在本文中，我们用微吸管给动脉施加负压使之变形的技术对动脉的弹性面积压缩性质进行了研究。以兔的胸主动脉为样本进行的实验表明动脉的表面积在微吸管负压作用下的变化与所加负压值可由指数关系Ｐ＝Ｋ（ｅｃａ－１）来拟合，其中Ｋ值反映了动脉壁的弹性面积压缩能力，称为弹性面积压缩模量，实验结果表明其正常值在５～１７ｋＰａ之间，而病态（动脉粥样硬化）下在３～１０ｋＰａ之间。实验证明了微吸管技术用于动脉力学实验的可行性，为在体血管力学性质的研究提供了有价值的技术手段。

he study of mechanical properties of aorta was important both in analysis of physiological and pathophysiological changes. In literature, experimental technique such as elongation and pressurization were applied to the aortas. In this paper, we attempted to apply the micropipette technique to study the elastic area compressibility of aorta. Using rabbit thoracic aortas as samples. The results showed that the relationship between the area change of the aorta surface and the negative pressure applied by the micropipettes could be regressed as exponential function, and the regressed constant K demonstrated the elastic area compressibility of aorta so we called it elastic area compressibility modulus. The experimental results showed the value was 517kPa for normal aorta and 310kPa for arteriosclerotic aorta. The micropipette technique was also feasible in the research of mechanical properties of aorta of in vivo.