6月龄苏尼特羊小肠黏膜结构及上皮内淋巴细胞和杯状细胞的分布

Mucosa Construction of Small Intestine from Sunit Goat at Six Month Age and Distribution of Intraepithelial Lymphocyte and Caliciform Cell

应用组织学和组织化学方法研究了6月龄苏尼特羊小肠黏膜结构、肌层厚度及黏膜上皮内淋巴细胞和杯状细胞分布数量的变化规律。结果显示,肠绒毛长度以十二指肠最长,为(353.25±60.48)μm,其次为空肠(317.27±72.02)μm和回肠(275.36±47.62)μm,且空肠和回肠之间差异显著(P<0.05);黏膜厚度以十二指肠最厚,为(669.15±139.04)μm,空肠和回肠分别为(586.72±134.50)和(551.85±91.32)μm,十二指肠与后两者间差异极显著(P<0.01);肌层厚度以回肠最厚,为(280.45±58.33)μm,与空肠(167.16±42.63)μm和十二指肠(148.78±38.36)μm相比,差异极显著(P<0.01);隐窝深度以十二指肠最深,为(309.36±74.21)μm,其次为空肠(286.23±57.23)μm,回肠最浅,为(237.83±48.86)μm,相互之间差异极显著(P<0.01);十二指肠和空肠的V/C比值分别为(1.80±0.45)和(2.00±0.48),空肠和回肠(1.39±0.34)相比,差异显著(P<0.05);小肠各段黏膜上皮内淋巴细胞和杯状细胞数量间无显著差异(P>0.05)。结果显示,在小肠各段,肠绒毛长度、黏膜厚度和隐窝深度由前向后逐渐变小而肌层厚度逐渐变大;黏膜上皮内淋巴细胞和杯状细胞数量间无显著差异(P>0.05)。

In the study,small intestinal mucosal structure,muscular layer thickness and the distributions and numbers of intraepithelial lymphocytes（IELs） and goblet cells（GCs） from Sunit sheep at 6 months old age was determined by Histological and Histochemical methods.The results showed the length of Duodenum villus were the longest,its length attained（353.25±60.48） μm,the Jejunum and Ileum in villus length were（317.27±72.02） μm and（275.36±47.62） μm respectively,but there was markedly different between Jejunum and Ileum（P0.05）;In mucosal thickness,Duodenum were the thickest reaching（669.15±139.04） μm,the Jejunum and Ileum were（586.72±134.50） μm and（551.85±91.32） μm,Duodenum in thickness was extremely different comapared with that of the Ileum and Jejunum（P0.01）;In the thickness of muscular layer,Ileum was the thickest reaching（280.45±58.33） μm,Jejunum was（167.16±42.63） μm and Duodenum was（148.78±38.36） μm,the difference was marked among them（P0.01）;In the depth of crypt,Duodenum was the deepest reaching（309.36±74.21） μm,Jejunum was（286.23±57.23） μm,the difference was marked among them（P0.01）;In the value of V/C,Duodenum and Jejunum were（1.80±0.45） and（2.00±0.48） respectively,there was different between Jejunum and Ileum（P0.01）;In the number of IELs and GCs,all parts of intestinal mucosal layer were not significantly different（P0.05）.The conclusion could be made that the length of villus,the mucosal thickness and the depth of crypt were gradually become smaller,while the muscular layer thickness were gradually become greater in small intestine from anterior to posterior,but there were no change of IELs and GCs numbers in all parts of small intestine.