淀粉水解程度对土曲霉发酵生产衣康酸的影响

Effect of starch hydrolysis degree on itaconic acid production by Aspergillus terreus

将玉米淀粉进行酸水解和酶水解,水解液作为土曲霉At394的碳源进行发酵。水解程度直接影响到衣康酸产量,适宜水解糖的DE值为35%。发酵培养基配方为淀粉液化水解糖(DE值为35%)100g/L,NH4NO33g/L,MgSO4.7H2O4g/L,玉米浆2g/L,KH2PO40.2g/L,pH3.0。在最适条件下,摇瓶发酵衣康酸产量可达54.1g/L,糖酸转化率为59.2%。

Corn starch, either acidic or enzymatically hydrolyzed, were used as substrates for itaconic acid production by Aspergillus terreos At394. The degree of hydrolysis had a great influence on itaconic acid production and the results showed that it was suitable when corn starch was sacchaff- fled at 35% DE （dextrose equivalent）. The medium composition was （g/L） 100 starch hydrolysate, 3 NH4NO3, 4 MgSO4＂TH20, 2 corn steep liquor, 0.2 KH2PO4 and pH 3.0. When the optimal conditions were applied in shaking flasks, the itaconic acid production was 54.1 g/L with a yield of 59.2 %.