一株产淀粉酶海洋菌的筛选及发酵条件的研究

Screening,optimization of fermentation conditions of an amylase-producing marine bacterium

研究筛选产淀粉酶海洋菌并对发酵条件进行了优化。采用3，5-二硝基水杨酸试剂显色法对菌种复筛；研究了碳源、氮源、各种理化因素等培养条件对细菌产淀粉酶的影响。确定W11菌为研究菌，初步鉴定为弧菌；最佳碳源为麸皮，最佳氮源为蛋白胨，最适初始pH值为7．04~7．70。培养温度、接种量、装液量对细菌产酶有较大影响，正交试验表明：麸皮10g／L，蛋白胨10g／L，250mL三角瓶中装液75mL，温度33℃时，酶活可达到325．14U／mL。比未优化前提高了2．71倍。

Screening of marine bacterium and optimization of fermentation conditions for amylase production were studied. The strain was finally de- termined with chtomogenic method with 3,5-dinitrosalicylic acid and effects of carbon source, nitrogen source, and physicochemical factors on pro- duction of amylase by bacterium were also studied. Strain Wl 1 was used for further study and it was preliminarily identified as Vibrionaceae. Opti- mum sources of carbon and nitrogen were wheat bran and peptone, respectively with initial pH at 7.04-7.70. With orthogonal test, the optimum con- ditions were determined as bran 10 g/l, peptone 10 g/l, with 75 ml medium in 250 ml shaking flask and inoculated under 33℃. Under the optimum conditions, enzyme activity could reach 325.14 U/ml, which was 2.71 times as normal conditions.