摘要
建立测定玉米浆中游离氨基酸的反相高效液相色谱法。DiamonsilCl8（2）反相柱（5μm，250mm×4．6mm）：梯度洗脱，流动相A相：0．05mol／L醋酸钠缓冲溶液（pH6．50），B相：乙腈-水（v／v=1：1），流速1．0mL／min，检测波长360nm。在该条件下所得各氨基酸的线性回归方程相关系数均在0．999以上。测定17种氨基酸的加样回收率94％-105％，相对标准偏差小于2．0％。
Method of determination of flee amino acids in corn syrup with RP-HPLC was established. The detection conditions were： using re- versed-phase column Diamonsil C18 （2） （5 μn, 250 min×4.6 mm）; gradient elution and mobile phase A： 0.05 mol/L sodium acetate buffer solution （pH = 6.50）; phase B： methyl cyanide ： water by 1：1 （V/V）, flow rate： 1.0 ml/min, detection wavelength： 360 nm. Under the conditions, correlation coefficients of linear regression equations of amino acids could reach above 0.999. Recovery of 17 kinds of amino acids determination was in the be- tween 94-105% and relative standard deviation was less than 2.0%. The method is simple and reliable. It can be used in determination office amino acids in corn syrup for antibiotic fermentation.
出处
《中国酿造》
CAS
北大核心
2010年第5期156-159,共4页
China Brewing
基金
国家科技支撑计划课题资助(2007BAI26B02)
