骨髓间充质干细胞对损伤卵巢的修复作用

Therapeutic effects of mesenchymal stem cell on injured ovary in mice

目的:探讨骨髓间充质干细胞移植对损伤卵巢的修复作用。方法:通过贴壁筛选法培养、纯化GFP转基因小鼠MSCs。将健康的雌性ICR小鼠(6 w)随机分为4组:正常对照组(A组,n=6)、MSCs移植组(B组,n=6)、MSCs治疗组(C组,n=6)、损伤组(D组,n=6)。B组腹腔移植MSCs,C、D组小鼠卵巢在无菌条件下,用体积分数为10%H2O2损伤卵巢建立损伤模型,24 h后C组腹腔移植MSCs,D组注射等体积的PBS。各组每周取卵巢,采用外观拍照、PCR检测GFP基因和病理切片观察MSCs是否对损伤卵巢有修复作用。结果:PCR结果表明MSCs经腹腔移植可到达正常卵巢,也可到达受损伤卵巢;C组移植MSCs后小鼠卵巢恢复的比D组快。结论:MSCs通过腹腔移植可到达卵巢,并可促进损伤卵巢的修复。

Aim：To explore the effects of bone mesenchymal stem cells（BMSCs） transplantation on injured ovary in mice. Methods： BMSCs were isolated from transgenic mice that ubiquitously expressed green fluorescent protein（GFP）,cultured and purified in vitro using their adherent characteristics.ICR mice（six weeks old） were divided into 4 groups randomly： normal control（A,n=6）,MSCs transplantation group（B,n=6）,MSCs treatment group（C,n=6） and ovary injury group（D,n=6）.MSCs were transplanted into the mice of group B though intraperitoneal injection.Ovaries of group C and group D were injured with 10% H2O2 under sterile conditions.Then MSCs or the same volume of PBS were transfused into the mice of group C or group D through intraperitoneal injection respectively 24 hours later.The ovaries of mice were observed weekly.Various methods,including visual observation,PCR for detection of GFP gene expression and pathological section analysis were carried out to determined whether MSCs can restore the damage of ovary.Results： The results of PCR showed that MSCs could reach the normal ovaries and the injured ovaries through intraperitoneal transplantation.The ovaries of mice from group C were recovered faster than that from the group D.Conclusion：MSCs can reach ovaries through intraperitoneal transplantation and restore the damage of the ovary.