河北省绿子叶黑豆种质资源表现型和ISSR标记遗传多样性分析

Genetic Diversity Analysis of Green-Cotyledon Black Soybean Germplasm Resources in Hebei Province based on Agronomic Traits and ISSR Markers

为了揭示河北省绿子叶黑豆种质资源的遗传多样性,为其研究利用提供理论根据,以46份原产河北省的绿子叶黑豆种质资源为试验材料,对其基于表型性状及ISSR标记鉴定结果进行了聚类分析,结果表明:7个ISSR引物共检测出60个等位变异,平均每个位点有8.6个等位变异,变幅为5～17个;ISSR引物的多态性信息量PIC变幅为0.721～0.927,平均0.820;利用表型性状和ISSR标记数据进行品种间遗传多样性分析,遗传相似系数变化范围分别为0.07～0.53和0.43～1.00,平均为0.284和0.704,遗传相似性变幅较大,河北省不同绿子叶黑豆品种间存在着丰富的遗传多样性。聚类结果显示,类群与品种来源地有关。

The genetic diversity of green-cotyledon black soybean cultivars native to Hebei province was evaluated based on ISSR and agronomic characters.The results indicated that 60 alleles of 7 ISSR markers were detected among 46 cultivars with an average of 8.57 alleles per ISSR marker,and the number of alleles per primer ranged from 5 to 17.The value of allelic polymorphism information content（PIC） ranged from 0.721 to 0.927,on the average of 0.82 per primer.The value of genetic similarity（GS） indices of 46 cultivars based on the 14 agronomic traits and ISSR data varied from 0.067 to 0.533 and 0.43 to 1.0 respectively,with an average of 0.284 and 0.704,indicated that the genetic diversity among green-cotyledon black soybean cultivars from Hebei was abundant.The clustering results showed that the groups could reflect the origin of the cultivars.