利用AFLP标记分析皮燕麦种质资源遗传多样性

Assessment of Genetic Diversity for Hulled Oat Germplasm Using AFLP Markers

用20对AFLP引物对来自于国内外的177份皮燕麦(Avena.sativa L.)资源进行遗传多样性分析,共获得976条清晰条带,其中多态性条带为185条,不同引物的多态性百分率为9.3%～35.9%,平均为19.0%。不同来源组群的Shannon-Weaver多样性指数变化范围为0.19～0.3412,西欧材料最高,其次是北欧(0.3269)、日本(0.3072)、东欧(0.2949)、北美(0.2904)、黑龙江最低。主坐标和UPGMA聚类分析结果基本相同,并与地理来源有很高的一致性。全部材料总体上可分为两类,其中一类全部为国内资源,另一类包含所有国外、内蒙古和青海的材料。国内与国外材料分布相对集中,这表明国内与国外材料亲缘关系较远,交流不是很广泛。而国内不同来源的材料相互交错分布,表明国内皮燕麦资源交流充分,多样性不是很丰富,应加强国外皮燕麦的引种工作。

One hundred and seventy seven accessions of hulled oat（Avena sativa L.）with domestic and oversea origins were analyzed using 20 AFLP primer combinations.Selective amplification created 976 unambiguous bands,of which 185 bands were polymorphic.The percentage of polymorphic bands for each AFLP primer combination ranged from 9.3% to 35.9%,with the average of 19.0%.Shannon-Weaver indices of groups of accessions with diverse geographic origins varied from 0.19 to 0.3412.Western Europe group showed the highest index,followed by North Europe（0.3269）,Japan（0.3072）,Eastern Europe（0.2949）and North American group（0.2904）,and Heilongjiang group showed the lowest.The results of PCA and UPGMA clustering analysis exhibited a good consistency,and were also consistent with geographic origins.All the accessions were classified into two categories based by PCAand UPGMA clustering analysis.One category covered mainly domestic accessions,the other category included accessions of Inner Mongolia and Qinghai as well as accessions from overseas.The exotic accessions were generally separated from domestic accessions while domestic accessions with different origins mixed up.This indicates that the relationship between domestic and oversea accessions was relatively far caused by lacking of effective exchange,and the domestic accessions of hulled oat were more homologous due to lack of diversity.It is important to promote the germplasm exchange with other countries and enrich the genetic diversity of hulled oat in China.