合并前列腺炎的前列腺增生组织中COX-2、VEGF的表达

Expression of COX -2 and VEGF in benign prostatic hyperplasia combined with prostatitis

目的研究环氧化酶-2（COX-2）、血管内皮生长因子（VEGF）在合并前列腺炎的前列腺增生组织中的表达及分析它们的相互关系，探讨前列腺炎对前列腺增生影响的可能机制。方法收集60例经尿道前列腺电切术（TURP）的前列腺增生患者的前列腺标本，常规HE染色后，光镜下观察其合并前列腺炎情况，根据是否合并前列腺炎，将标本分为单纯前列腺增生组（A组）和合并前列腺炎的前列腺增生组（B组），采用免疫组织化学和实时荧光定量PCR（RT—PCR）检测环氧化酶-2（COX-2）和血管内皮生长因子（VEGF）在前列腺组织中的表达情况。结果（1）免疫组织化学结果：COX-2、VEGF在A组和B组组织中都有表达，且在B组组织中表达均明显高于A组组织中的表达（P〈0．05）；COX-2和VEGF在B组组织中表达有正相关性（R=0．92，P〈0．05）（2）COX-2mRNA在A组中的相对比值：0．141±0．019，在B组中相对比值：0．161±0．013，VEGFmRNA在A组中的相对比值：0．254±0．015，在B组中的相对比值：0．341±0．012，COX-2mRNA、VEGFmRNA在B组组织中的表达均明显高于A组组织中的表达（P〈0．05）。结论炎症因子COX-2和增生因子VEGF在合并前列腺炎的前列腺增生组织的表达明显上调且两者表达水平有正相关性，COX-2可能通过上调VEGF的表达参与前列腺增生的发展进程。

Objectives To study the expressions of Vascular endothelial growth factor （VEGF） and cyclooxygenase -2 in benign prostatic hyperplasia combined with Prostatitis , analyze their correlation and to investigate the effect and molecular mechanism of prostatitis on BPH. Methods 60 specimens were obtained from patients undergoing transurethral prostatic resection（TURP）. The paraffin section of the specimens were stained with hematoxylin and eosin, and observed under light microscope to examine the inflammation pathological changes. Depending on whether the benign prostatic hyperplasia combined with prostatitis, those specimens were divided into group of simple benign prostatic hyperplasia （ A group ） and group of benign prostatic hyperplasia combined with prostatitis （ B group）, using immunohistochemistry and Real - time fluorescence quantitative PCR （ RT - PCR） to detect the expressions of COX - 2 and VEGF in prostate tissues. Results （ 1 ） immunohistochemical staining： COX - 2 and VEGF expressed in the A group and B group, but in the B group of tissues were significantly higher than A group of tissues（ p 〈 0.05 ） ; COX - 2 and VEGF expression in the B group of organizations had a positive correlation （R = 0.92, p 〈0.05） （2） COX -2 mRNA in the A group, the relative ratio： 0. 141 ± 0.019,in the B group, the relative ratio： 0. 161 ±0.013;VEGF mRNA in the A group relative ratio： 0.254 ± 0.015, in the B group, the relative ratio ： 0. 341 ± 0. 012, The expressions of COX - 2mRNA and VEGF mRNA in the B group were significantly higher than the expressions of A group （ p 〈 0.05 ） . Conclusions The inflammatory factor COX - 2 and prolif- erative factor VEGF in the tissues of benign prostatic hyperplasia combined with prostatitis are significantly increased and the expression levels of both positive correlation, COX - 2 by the way of up - regulating VEGF expression may be involved in the development process of benign prostatic hyperplasia.