磺胺二甲嘧啶在鲫鱼体内的药代动力学研究

A Study of Pharmacokinetics Property of Sulfadimidine in Crucian Carp

为考察磺胺二甲嘧啶(SM2)在鲫鱼(Carassius auratus)体内的药代动力学过程,按200 mg.kg-1的剂量给鲫鱼口灌SM2后,用高效液相色谱(HPLC)法测定鲫鱼血液、肝脏和肌肉组织中的药物浓度,用MCPKP药代动力学软件处理药时数据。结果表明,SM2在鲫鱼体内的药动学最佳数学模型为一级速率吸收一室开放模型,主要动力学参数中,吸收半衰期(T(1/2)Ka)为0.782 h,半衰期(T(1/2)K)为14.834 h,最高血药浓度(Cmax)为75.596μg.mL-1,血药浓度时间曲线下面积(AUC)为137.82 mg.h.L-1;根据SM2的药代动力学规律和抗菌药物的应用原则制定用药方案:口灌200 mg.kg-1剂量的SM2,每日给药1次,5 d一个疗程为宜。本研究为水产养殖中确定合理的SM2用药方案提供了可靠的理论依据。

In the using of high efficient liquid chromatography, the pharmacokinetics processes properties of Sulfadimidine is determined in a local fish, crucian carp, after oral administration. The experimental fish is fed with Sulfadimidine at a dose of 200 mg/kg. The concentrations of Sulfadimidine in the blood., liver, and muscle of the crucian carp are measured by the high efficient liquid chromatography. The data are conducted by the software of MCPKP and the parameters of the pharmacokinetics function are calculated. The results show that Sulfadimidine concentrations versus time conform to the open dynamic model of one compartment after a single meal administration. Absorption half-life （T（1/2）Ka） is 0. 782 h, elimination half-life （Ti1/2）K） is 14. 834 h,and maximum concentration （ Cmax ） is 75. 596μg/mL,and area under concentration （AUC） is 137.82 （ mg · h） /L. According to the pharmacokinetics properties of SM2 the medicine ration is defined as 5 days SM2 oral conduction at the dose of 200 mg/kg/day, which would be optimum. This study has observed the pharmacokinetics properties of Sulfadimidine in the crucian carp, which could supplies some basic data for medical ration in aquaculture.