海洋真菌Nigrospora sphaerica化学成分的分离与鉴定(Ⅰ)

Isolation and identification of chemical constituents from the marine Nigrospora sphaerica(Ⅰ)

目的对海洋真菌Nigrospora sphaerica中的化学成分进行研究。方法采用硅胶柱色谱、凝胶以及HPLC等方法进行分离纯化,根据理化性质和光谱数据进行结构鉴定。结果分离得到10个化合物,分别鉴定为尿嘧啶（uracil,1）、胸腺嘧啶（thymine,2）、半乳糖醇（D-galactitol,3）、5α,8α-过氧麦角甾-6,22E-二烯-3β-醇（5α,8α-epidioxy-（22E,24R）-ergosta-6,22E-dien-3β-ol,4）、5α,8α-过氧麦角甾-6,9,22E-三烯-3β-醇（5α,8α-epidioxy-（22E,24R）-ergosta-6,9,22E-triere-3β-ol,5）、丁二酸（succinic acid,6）、4-（2-羟乙基）苯酚（4-（2-hydroxyethyl）phenol,7）、3-异丁基-7-羟基-吡咯并哌嗪-1,4-二酮（3-isobutyl-7-hydroxy-pyrrolopiperazine-1,4-dione,8）、对羟基苯甲酸（4-hydroxybenzoicacid,9）和苯乙酸（phenylacetic acid,10）。结论海洋真菌Nigrospora sphaerica为首次从海洋中分离得到,化合物4~10为首次从该真菌中分离得到。

Objective To study chemical constituents from the marine Nigrospora sphaerica.Methods The compounds were isolated by chromatography on silica gel columns,Sephadex LH-20 and HPLC,and then identified by their physical-chemical properties and spectral analysis.Results Ten compounds were obtained and identified as uracil（1）,thymine（2）,D-galactitol（3）,5α,8α-epidioxy-（22E,24R）-ergosta-6,22E-dien-3β-ol（4）,5α,8α-epidioxy-（22E,24R）-ergosta-6,9,22E-triere-3β-ol（5）,succinic acid（6）,4-（2-hydroxyethyl）phenol（7）,3-isobutyl-8-hydroxy-pyrrolo-piperazine-1,4-dione（8）,4-hydroxybenzoic acid（9）,phenylacetic acid（10）,respectively.Conclusions The Nigrospora sphaerica is obtained from marine for the first time,compounds 410 are isolated from the marine fungi for the first time.