西洋参各部位皂苷成分的HPLC测定

Determination of Ginsenosides for diferent parts in Panax quinquefolium L. by HPLC

采用索氏技术提取样品液,HPLC法测定西洋参中6种主要单体皂苷Rg1、Re、Rb1、Rc、Rb2、Rd的含量。比较分析西洋参各部位总皂苷及单体皂苷的含量差异。色谱条件:Hypersil-C18柱(4.6 mm×250 mm,5μm),水和乙腈梯度洗脱,流速1.0 mL/min,柱温35℃,检测波长203 nm,进样量20μl。在选定的色谱条件下,各单体皂苷在其浓度范围内线性关系较好。测定结果表明,西洋参各部位皂苷成分的含量存在一定差异。该方法分析人参皂苷成分简捷有效。适用于西洋参不同样品材料的人参皂苷含量分析。

Panax quinquefolium L. was extracted with Soxhelt Extraction and determined the contents of ginsenoside Rg1, Re, Rb1, Rc, Rb2, Rd by high performance liquid chromatography （ HPLC ） . Then, compared the different contents of total saponins and monomer saponins in different parts of Panax quinquefolium L. Agilent 1100 Series HPLC instrument with the condition of Hypersil- C18 column（4.6 mm ×250 mm,5 μm） ; acetonitrile and water was used as the mobile phase and eluted in gradient mode at a flow rate of 1.0 mL· min^- 1 ; column temperature at 35 ℃ ; the warelength of detector was 203 nm ; injection volume was 20 μl. Under the selected chromatographic conditions, the ginsenosides had a good linearity over their respective concentration range. The results show the contents of ginsenosides in different parts of Panax quinquefolium L. had a significant variation. The method is simple, fast and accurate. It can be used to determinate the contents of ginsenosides for different samples of Panax quinquefolium L.