乙肝患者联合检测血清前S_1、S_2抗原的临床意义

Clinical significance of detection HBV Pre-S1 and Pre-S2 antigens for the patients with hepatitis B virus infection

目的研究联合检测乙肝患者前S1、S2抗原的临床意义。方法采用ELISA双抗体夹心法检测300例乙肝患者血清HBsAg、前S1抗原(Pre-S1Ag)及前S2抗原(Pre-S2Ag),荧光定量PCR方法检测其HBV DNA的含量,并采用速率法检测患者血清谷丙转氨酶(ALT)的水平。结果 300例乙肝患者Pre-S1Ag阳性组、Pre-S2Ag阳性组与Pre-S1Ag+Pre-S2Ag阳性组的例数与HBV DNA阳性组相比较没有统计学差异。Pre-S1Ag与Pre-S2Ag双阳性组中HBV DNA阳性的比例显著高于Pre-S1Ag阳性组和Pre-S2Ag阳性组。Pre-S1Ag+Pre-S2Ag阳性组中ALT异常例数的比例显著高于Pre-S1Ag阳性组和Pre-S2Ag阳性组。结论联合检测Pre-S1Ag和Pre-S2Ag对HBV感染的诊断、了解HBV的复制以及检测患者的肝功能具有重要的临床意义。

Objective To study the clinical significant of detection HBV Pre-S1 and Pre-S2 antigens for the patients of type B hepatitis.Methods For the 300 patients with hepatitis B virus infection,detection of HBV Pre-S1 antigen and Pre-S2 antigen with ELISA and the levels of alan ine am inotransferase（ALT） were measured by an automated biochemistry analyzer.Results No significant difference was found among the detectable rates of HBV DNA,HBV Pre-SlAg,HBV Pre-S2Ag and Pre-S1Ag＋Pre-S2Ag in patients with hepatitis B virus infection.In the both Pre-S1Ag and Pre-S2Ag positive group,the detectable rates of HBV DNA were significant higher than the Pre-S1Ag-positive group and the Pre-S2Ag-positive group.The detectable rates of patients with high levels of ALT in the both Pre-S1Ag and Pre-S2Ag positive group were significant higher than the Pre-S1Ag-positive group and the Pre-S2Ag-positive group.Conclusion Detection of HBV Pre-S1 and Pre-S2 antigens together have important clinical significances in diagnosing infection of HBV and analyzing situation of reproduction of HBV and observing the liver function.