多溴联苯醚对HL-7702细胞和小鼠皮肤成纤维细胞间隙连接通讯的影响

Effects of polybrominated diphenyl ethers on gap junctional intercellular communication in HL-7702 cells and mouse skin fibroblasts

利用划痕染料示踪标记(SLDT)方法,检测了多溴联苯醚(PBDEs)对人肝正常细胞(HL-7702)与小鼠皮肤成纤维细胞间隙连接通讯(GJIC)的影响.结果表明,PBDE-47与PBDE-209均显著抑制HL-7702细胞的GJIC(10～50μmol.L-1),随着剂量的升高,抑制作用随之增强.在小鼠皮肤成纤维细胞中,PBDE-47在3.75～50μmol.L-1范围内对GJIC有显著的抑制作用,而PBDE-209在15～50μmol.L-1范围内对GJIC有显著的抑制作用,在两种细胞中,PBDE-47的抑制作用大于PBDE-209.停止染毒后,细胞GJIC有一定程度的恢复.实验表明PBDEs可抑制细胞间隙连接通讯,为进一步研究PBDEs的毒性机制提供了科学依据.

The effects of polybrominated diphenyl ethers（PBDEs） on gap junctional intercellular communication（GJIC） in human liver HL-7702 cells and mouse skin fibroblasts are investigated by using scrape loading and dye transfer（SLDT） method.The results show that both PBDE-47 and PBDE-209 could inhibit the GJIC in human liver HL-7702 cells significantly at 10～50 μmol·L-1 with a dose-dependent manner.In mouse skin fibroblasts,PBDE-47 inhibite GJIC at 3.75-50 μmol·L-1 and PBDE-209 at 15～50 μmol·L-1.The inhibition efficiency of PBDE-47 is higher than PBDE-209 in both cell lines.The recovery of GJIC is observed after removal of tested chemicals.These results suggest PBDEs can inhibit GJIC,which provides scientific basis for future study about mechanism of toxicity induced by PBDEs.