连锁群选育法及其在疟原虫研究中的应用

Linkage Group Selection and Its Application in Malaria Parasites

鉴定控制耐药性、生长速率以及株特异性免疫力等重要表型的基因,对于控制疟疾具有重要意义,而传统的遗传学方法很难达到该目的。论文介绍一种鉴定表型相关基因的新型遗传学方法——连锁群选育法(linkage group selection,LGS),该方法具有快速、有效、省力等优点。采用LGS已成功鉴定了与疟原虫耐药性、株特异性免疫力等表型相关的靶基因。由于泰勒属(Theileria)、巴贝斯属(Babesia)、弓形虫属(Toxoplasma)、艾美耳属(Eimeria)等顶复器原虫在生活史中与疟原虫具有相同的遗传学特征,故LGS也有望应用于这些原虫相关表型基因的研究。

The identification of parasite genes controlling phenotypes such as drug resistance,virulence,immunogenicity,and transmission is immense importance in the fight against malaria.Classical genetic methods have achieved these goals only rarely and with difficulty.We describe here a novel genetic method,Linkage Group Selection（LGS）,which achieves rapid de nove location of genes encoding selectable phenotypes of malaria parasites.LGS has enabled to locate genes controlling drug sensitivity and strain-specific immunity of the parasites.Because of the equivalence of the genetic features of their life cycles,LGS will be equally applicable to other Apicomplexan parasites,including such important pathogens as the Theilerias,Babesias,and Eimerian parasites of domestic animals.