大孔树脂AB-8动态吸附黄柏总生物碱的工艺研究

Research on Dynamic Adsorption Technology of Total Alkaloids from Cortex Phellodendri with AB-8 Macroporous Resin

【目的】考察大孔树脂AB-8对黄柏总生物碱动态吸附和解吸的最佳工艺条件。【方法】采用AB-8树脂对黄柏提取液中总生物碱进行动态吸附,以总生物碱的吸附量或解吸量为指标,分别考察了上样量、流速、杂质洗脱剂水的用量、洗脱剂的浓度和用量。【结果】动态吸附的较佳工艺条件为:每mL树脂上样50 mL样品,上柱流速20 mL(2个柱体积)/h,杂质洗脱剂水的用量为30 mL(3个柱体积),洗脱剂采用体积分数40%的乙醇,用量为60 mL(6个柱体积),在此工艺条件下操作,得到总生物碱的纯度为40.06%,比上柱之前的纯度提高了2.56倍。【结论】该法简便易行,纯化效果好。

Objective To optimize the adsorption and desorption conditions for total alkaloids from Cortex Phellodendri with AB-8 macroporous resin.Methods With the adsorption and desorption volume of total alkaloids as the observation indexes,the experiment of dynamic adsorption with AB-8 macroporous resin was carried out to observe the effect of the sample volume,flow rate,water volume for removing impurity,and the concentration and volume of eluant on the total alkaloids from Cortex Phellodendri.Results The optimal adsorption conditions were as follows：sample volume being 50mL for each milliliter of the resin,flow rate being 20mL/h（equal to 2 column volume）,water for impurity eluent being 30 mL（equal to 3 column volume）,and the eluent being 60 mL（equal to 6 column volume） of 40% ethanol.Under the above condition,the purity of the total alkaloids was 40.06%,which is 2.56 times as much as the total alkaloids before loading the column.Conclusion This methed is simple and shows good purifying effect.