持续游泳训练对大鼠杏仁核cAMP浓度的影响

The Influence of Endurance Swimming Exercise on the cAMP Contents in Amygdala of Rat

采用大鼠环磷酸腺苷cAMP ELISA法,检测持续游泳训练后大鼠杏仁核cAMP浓度变化,以探讨运动应激引起机体反应的机制。结果表明,持续游泳训练后,除1 h时cAMP浓度显著低于对照组外(P<0.01),即刻、0.5 h、2 h大鼠杏仁核cAMP浓度均显著高于对照组(P<0.05);训练后即刻,大鼠杏仁核cAMP浓度快速升高,至0.5 h时达到最高值,之后1 h时cAMP浓度急剧下降,2 h时其浓度回升,高于对照组的水平,随后4 h时下降至略低于对照组水平,但差异不显著。整个浓度变化曲线呈锯齿形。持续游泳训练后杏仁核cAMP浓度变化具有时相性;持续游泳训练可激活大鼠杏仁核环磷酸腺苷(cAMP)通路,进而影响机体的后续反应。

To detect the change of the cAMP contents in amygdale after exercise by using the method of ELISA,and explore the mechanism of the body＇s response by exercise stress.The result showed that：The cAMP contents in amygdale of rats at 0 h、0.5 h、2 h after endurance swimming has higher level than control group（P〈0.05）,besides the cAMP contents of the endurance group at 1h is lower than the control group（P0.01）;In amygdale,the cAMP contents rising rapidly and reach the peak at 0.5h after swimming finished,then the cAMP contents dropped at 1 h,the cAMP contents increased at 2 h,the level was higher than that of control group,and then dropped,the level was still lower than that of control group at 4 h after swimming finished.The change figure of cAMP contents appears the shape of sawteeth.It concluded that there must be a phasic change in the contents of cAMP.Endurance swimming could influence the cAMP signaling pathway in amygdale of rats with effecting the body＇s response.