FasL mRNA在慢性乙型肝炎肝组织中的表达

The localization of FasL mRNA in liver tissues of chronic hepatitis B

目的研究ＦａｓＬｍＲＮＡ在慢性乙型肝炎（ＣＨＢ）肝组织中的表达及意义。方法采用人工合成ＦａｓＬ寡核苷酸探针对ＣＨＢ肝组织进行原位杂交检测。结果肝组织浸润单个核细胞及肝细胞都不同程度检测出ＦａｓＬｍＲＮＡ，阳性细胞主要分布在碎屑样坏死、灶状坏死周围。ＦａｓＬｍＲＮＡ还可在窦细胞中表达。结论慢性乙型肝炎时肝细胞自身合成并表达ＦａｓＬ。同时ＦａｓＬ阳性细胞可能经Ｆａｓ机制发生“细胞自杀”或“同胞相杀”，这一机制在ＣＨＢ致病中可能起重要作用。结果还提示细胞毒性Ｔ淋巴细胞可能通过Ｆａｓ机制诱导感染的靶细胞凋亡并致慢性肝损害。

To study the expression of FasL mRNA in liver tissues of CHB, we investigated FasL mRNA by in situ hybridization in liver tissues from patients with chronic hepatitis B.FasL oligonucleotide probes were synthesized and labeled with DIG-Oligonucletide 3'-End Labeling Kit. FasL mRNA was found in cytoplasm of liver-infiltrating mononuclear cells, hepatocytes and some sinus cells. FasL mRNA-positive hepatocytes were present mainly at the edges of `piecemeal necrosis(PN)' and `bridging necrosis (BN)'. These results demonstrated that human hepatocytes themselves can express FasL in chronic hepatitis B.We deem that FasL-positive hepatocytes might express cytotoxic activity and eliminate Fas-positive hepatocytes by both `fratricide' and `suicide'. These results also emphasize that apoptosis caused by CTL through Fas-mediated pathway may play an important role in CHB.