艾灸三阴交穴缓解兔子宫痉挛的实验研究

Experimental Research on the Mitigation Effect of Moxibustion at Sanyinjiao Points on Uterine-spasm of the Rabbit

目的:探讨艾灸不同穴位对雌性家兔未孕的子宫痉挛性收缩频率、幅度和血清钙离子(Ca2+)、钾离子(K+)及血浆内皮素(ET)含量的影响,分析不同穴位对子宫肌痉挛的缓解作用及其部分机制。方法:通过注射缩宫素造成子宫痉挛模型,分别空白组、模型组、艾灸三阴交、足三里、非经非穴点。结果:艾灸三阴交穴组子宫肌活动参数恢复至注射药物缩宫素(OT)前水平,而其余各组未恢复,且三阴交穴组Ca2+水平明显高于其它两组,而K+明显低于其余两组,各组对NO、ET含量变化均未见统计学差异。结论:三阴交穴对子宫肌痉挛的缓解作用有一定的特异性,艾灸三阴交穴缓解痛经的作用机理是通过调节血清钙-钾平衡而达到的。

Objective：To observe the effect of moxibustion at different acupoints on frequency and amplitude of hysterospasm,content of Ca^2＋ and K^＋ in serum and ET in plasma in female Nonfertile rabbits,and to probe the mechanism.Methods：30 rabbits were randomly divided into 5 groups：blank group,model group,moxibustion at sanyinjiao points group;moxibustion at zusanli points group;moxibustion at non-acupoints.The animal model of uterine spasm was established by oxytocin injection.Results：After moxibustion at sanyinjiao point,the parameters of uterine movements reached the values before oxytocin injection.Compared with the other two moxibustion groups,the content of Ca^2＋ was significantly higher,while the content of K^＋ was significantly lower in sanyinjiao point group.The content of NO and ET had no significant difference among the groups.Conclusion：Moxibustion at sanyinjiao point could relieve uterine spasm.The mechanism of anti-spastic effect of moxibustion at sanyinjiao points is to regulate calcium-potassium balance in serum.