灌注动物细胞培养及其在组织工程中的应用进展

Advances in perfusion animal cell culture and its application in tissue engineering

灌注培养方法是在传统的培养方法基础上发展起来的新的动物细胞的培养方法。目前,组织工程方面有关灌注培养条件下细胞体外生长的研究表明,灌注培养提供的动态培养环境有利于细胞体外生长和增殖,可以得到与活体组织相似的体外组织,用灌注培养所得的细胞或组织进行体内移植的成功率较高。灌注培养方法为组织工程的研究和应用开辟了新的前景,本文对灌注动物细胞培养及其在组织工程中的应用研究进展进行综述如下。

Perfusion culture way is a new animal cell culture technique which comes from traditional culture methods.The present researches on tissue engineering of cell growth under perfusion culture have showed that the dynamic culture environment from perfusion culture is useful for the growth and differentiation of cells in vitro.The tissues and cells cultured in perfusion culture system show to be similar to nature tissues and cells,and these kinds of cells and tissues have higher successful rate for transplantation in vivo.Perfusion culture ways open up new prospects for tissue engineering researchs and applications,this article reviews the advances in perfusion animal cell culture and its application in tissue engineering.