缺氧诱导因子-1α在老年性痴呆模型鼠海马中的表达被引量：1

下载PDF

导出

摘要目的观察老年性痴呆(AD)大鼠行为学、海马神经细胞凋亡率及缺氧诱导因子(HIF)-1α表达水平的变化。方法侧脑室注射Aβ25～35构建AD大鼠模型,Morris水迷宫实验测试大鼠空间学习记忆能力,流式细胞术检测海马神经细胞凋亡率,Western印迹技术检测大鼠海马HIF-1α蛋白的表达。结果Morris水迷宫实验结果显示AD组大鼠平均逃避潜伏期显著延长(P<0.05),流式细胞分析结果显示,AD组海马神经细胞凋亡百分率较正常对照组显著升高(P<0.05),Western印迹检测结果显示,AD组海马神经细胞HIF-1α蛋白表达水平较正常对照组显著升高(P<0.05)。结论侧脑室注射Aβ25～35成功构建AD大鼠模型并上调海马神经细胞HIF-1α水平表达。

作者刘凌云马媛媛李欣柴锡庆

机构地区上海市杨浦区中心医院神经内科复旦大学上海医学院医学神经生物学国家重点实验室河北医科大学研究生学院

出处《中国老年学杂志》 CAS CSCD 北大核心 2010年第8期1104-1105,共2页 Chinese Journal of Gerontology

关键词阿尔茨海默病缺氧诱导因子-1Α Β-淀粉样蛋白海马

分类号 R388.2 [医药卫生—医学寄生虫学]

引文网络
相关文献

参考文献10

1王鑫,张莹,何金生,包福祥,洪涛.阿尔茨海默病的疫苗研究进展[J].中国老年学杂志,2009,29(12):1577-1580. 被引量：3
2Behl C,Davis JB,Lesley R,et al.Hydrogen peroxide mediates amyloid beta protein toxicity[J].Cell,1994;77(6):817-27.
3Sagara Y,Dargusch R,Klier FG,et al.Increased antioxidant enzyme activity in amyloid beta protein-resistant cells[J].J Neurosci,1996;16(2):497-505.
4Semenza GL.Regulation of mammalian O2 homeostasis by hypoxia-inducible factor 1[J].Annu Rev Cell Dev Biol,1999;15(5):551-78.
5张文亮,李荣亨.缺氧诱导因子-1的病理意义[J].中国老年学杂志,2008,28(12):1140-1142. 被引量：2
6Soucek T,Cumming R,Dargusch R,et al.The regulation of glucose metabolism by HIF-1 mediates a neuroprotective response to amyloid beta peptide[J].Neuron,2003;39 (1):43-56.
7Kaelin WG,Ratcliffe PJ.Oxygen sensing by metazoans:the central role of the HIF hydroxylase pathway[J].Mol Cell,2008;30(4):393-402.
8Parihar MS,Hemnani T.Alzheimer′s disease pathogenesis and therapeutic interventions[J].J Clin Neurosci,2004;11(5):456-67.
9Erler JT,Cawthorne CJ,Williams KJ.Hypoxia-mediated down-regulation of Bid and Bax in tumors occurs via hypoxia-inducible factor 1-dependent and -independent mechanisms and contributes to drug resistance[J].Mol Cell Biol.2004;24(7):2875-89.
10Sasabe E,Tatemoto Y,Li D.Mechanism of HIF-1alpha-dependent suppression of hypoxia-induced apoptosis in squamous cell carcinoma cells[J].Cancer Sci,2005;96(7):394-402.

二级参考文献47

1李少兵,汪华侨,林贤,徐杰,姚志彬.接种Aβ_(42)全肽疫苗恒河猴的特异性体液免疫应答[J].细胞与分子免疫学杂志,2005,21(2):202-204. 被引量：6
2李少兵,汪华侨,林贤,徐杰,姚志彬.Aβ_(1-15)疫苗免疫接种成年恒河猴的体液免疫效应[J].中国病理生理杂志,2005,21(4):761-764. 被引量：8
3洪欣,董宏彬,杜宏伟,尹昭云.低氧及低氧预处理时心肌细胞HIF-1α与凋亡相关蛋白表达的变化[J].中国应用生理学杂志,2005,21(4):423-426. 被引量：6
4张蓓,吴海琴,张海雄,张桂莲,展淑琴.低氧诱导因子-1、促红细胞生成素在血管性痴呆大鼠海马的表达[J].四川大学学报（医学版）,2006,37(5):730-733. 被引量：9
5Walsh DM, Klyubin I, Fadeeva JV, et al. Naturally secreted oligomers of amyloid beta protein potently inhibit hippocampal long-term potentiation in vivo[J]. Nature, 2002 ;416(6880) :535-9.
6Schenk D, Barbour R, Dunn W, et al. Immunization with amyloid-β attenuates Alzheimer-disease-like pathology in the PDAPP mouse[J]. Nature, 1999 ;400 ( 6740 ) : 173-7.
7Janus C, Pearson J, McLaurin J, et al. Aβ peptide immunization reduces behavioural impairment and plaques in a model of Alzheimer's disease[ J]. Natrue, 2000 ;408 (6815) :979-82.
8Morgan D, Diamond DM, Gottschall PE, et al. Aβ peptide vaccination prevents memory loss in an animal model of Alzheimer's disease[ J]. Nature, 2000 ;408 (6815) :982-5.
9Weiner HL, Lemere CA, Maron R, et al. Nasal administration of amyloid-beta peptide decreases cerebral amyloid burden in a mouse model of Alzheimer's disease[J]. Ann Neurol, 2000;48(4) :567-79.
10Das P, Murphy MP, Younkin LH, et al. Reduced effectiveness of Aβ1-42 immunization in APP transgenic mice with significant amyloid deposition [J]. Neurobiol Aging, 2001 ;22(5) :721-7.