植入用缓释氟尿嘧啶治疗结直肠癌的实验研究

Effect of delayed-release 5-fluorouracil implants on colorectal cancer

目的 研究氟尿嘧啶（5-Fu）缓释植入剂对结直肠癌移植瘤的治疗效果.方法 将50只直肠癌荷瘤鼠随机分为5组,每组10只.A、B组于瘤周植入5-FU缓释剂,剂量分别为200mg/kg和100 mg/kg;C、D组于瘤周注射5-FU注射液,剂量分别为200 mg/kg和100 mg/kg;E组不予任何治疗.分别于给药后0、3、6、9和12 d,观察裸鼠生存情况、体质量变化及肿瘤体积,12 d后处死小鼠.结果 A、B组肿瘤生长曲线平缓,12 d后A、B、C及D组抑瘤率分别为72%、51%、8%及5%,A、B组肿瘤体积与C、D组比较,差异有统计学意义（P＜0.05）.A、B、C和D组在给药3 d后体质量下降,其中以C组最为明显;以后各组体质量增加,12 d后,各组小鼠体质量间差异无统计学意义（P＞0.05）.在实验过程中,A、B、C和D组小鼠分别死亡1、0、4和1只.结论 5-FU缓释植入剂于瘤周植入能安全有效地抑制结直肠癌移植瘤的生长.

Objective To study the antitumor effect of peri-tumor implantation of delayedrelease 5-fluorouracil implants on xenograft colorectal tumor in mice.Methods Fifty tumor-beating nude mice were randomly divided into 5 groups.Group A and B were treated with peri-tumor implantation of 5-fluorouracil implants and the dose of 5-fluorouracil was 200 and 100 mg/kg,respectively.Group C and D were treated with peri-tumor injection of 5-fluorouracil solution and the dose of 5-fluorouracil was 200 and 100 mg/kg,respectively.Group E did not receive any treatment.A growth curve was plotted for changes in tumor volume,the weight of the tumor was measured and tumor inhibition rate was calculated.Results The growth curve was mild in group A and B and steep in group C,D and E.There were statistical differences in tumor volume between groups A and B and other groups and there were no statistical differences in tumor volume among group C,D and E.After 12 days,tumor inhibition rate was 72% in group A,51% in group B,8% in group C,and 5% in group C.There were statistical differences in inhibition rate between group A,B and C,D （P〈0.05）.The weight changes before and after the treatment among the 5 groups were not statistically different.During the study,1 mouse in group A died,4 in group C and 1 in group D.Conclusion Delayedrelease 5-fluorouracil implants can effectively inhibit tumor growth.