摘要
目的探讨JAK2-STAT3-波形蛋白信号通路在结肠癌细胞增殖迁移中的作用。方法应用JAK2抑制剂AG490处理人结肠癌Lovo细胞株。采用MTT法进行细胞增殖实验：采用细胞划痕实验检测细胞迁移能力；用免疫荧光染色和Western blot检测Lovo细胞中波形蛋白和磷酸化STAT3（P—STAT3）的表达水平。结果AG490处理后，Lovo细胞增殖明显受到抑制．且呈剂量依赖性和时间依赖性（P〈0．05）。细胞划痕后24h，AG490处理组（实验组）的细胞划痕宽度恢复20％，而对照组划痕宽度恢复60％（P〈0．05）。实验组Lovo细胞中P—STAT3和波形蛋白蛋白表达明显降低（P〈0．05）。结论JAK2-STAT3-波形蛋白信号通路参与调控人结肠癌细胞的增殖和迁移。
Objective To investigate the regulatory role of JAK2/STAT3/vimentin signaling pathway on the proliferation and migration of human colon cancer cells. Methods The human colon cancer cell Lovo was treated with Janus kinase inhibitor AG490. The proliferation of Lovo cells was quantified by MTF assay. The migration of Lovo cells was measured with scratch assay. The intracellular phosphorylation STAT3 （P-STAT3） and vimentin protein was detected by Western blot and immunofluorescence. Results After AG490 treatment, the proliferative ability of Lovo cells decreased as compared with control group （P〈0.05）. This suppression was dose-independent and time-independent. At 24 hrs after scratching, scratch width in the AG490 group recovered to 20%, lower than that in the control group （60%,P〈0.05）. After AG490 treatment, the expression of P-STAT3 and vimentin in Lovo cells decreased sigificantly （P〈0.05）. Conclusion JAK2/STAT3/vimentin signaling pathway participates in regulating the proliferation and migration of human colon cancer cells.
出处
《中华胃肠外科杂志》
CAS
北大核心
2010年第4期282-285,共4页
Chinese Journal of Gastrointestinal Surgery
基金
全军医学科研“十一五”计划面上项目（06MB243）
重庆市自然科学基金（CSTC-2005BB5291）
