肝纤维化与肝硬化组织中趋化因子CXCL5和CXCL8的表达意义

Expression and significance of CXCL5 and CXCL8 in hepatic fibrosis and cirrhosis tissues

目的探讨肝纤维化与肝硬化组织中趋化因子CXC15和CXCL8的表达意义。方法收集2008年5月至2009年5月南方医科大学南方医院9例肝血管瘤患者、10例肝纤维化患者和11例肝硬化患者肝组织标本，采用ELISA法检测肝组织中的CXCL5和CXCL8的含量。采用单因素方差分析，双变量正态分布采用Pearson等级相关分析，不符合双变量正态分布的采用Spearman相关系数表示。结果肝血管瘤、肝纤维化、肝硬化患者肝组织中CXCL5的含量分别为（0．8±0．7）、（2．0±2．0）、（17．1±4．8）ng／g；CXCL8的含量分别为（6．2±3．7）、（11．6±3．5）、（12．3±3．9）ng／g；3者比较差异有统计学意义（F=60．050，7．690，P〈0．05）。CXCL5与ALT、AST、胛具有相关性（r=0．502，0．468，0．523，P〈0．05）；CXCL8与ALT、AST、TBil、PT具有相关性（r=0．477，0．504，0．537，0．431，P〈0．05）。结论肝脏发生纤维化损伤时CXCL5和CXCL8的含量均显著增高，但两者的变化规律不同。CXCL5和CXCL8的变化与肝脏损伤有关，但变化的程度与肝病的损害程度不完全一致。

Objective To investigate the level of chemotactic factors （ CXCL5 and CXCL8 ） in hepatic fibrosis and cirrhosis tissue. Methods Hepatic tissues were obtained from 9 patients with hepatic hemangioma （hepatic hemangioma group）, 10 patients with liver fibrosis （liver fibrosis group ） and 11 patients with liver cirrhosis （liver cirrhosis group） at Nanfang Hospital from May 2008 to May 2009. The contents of CXCL5 and CXCL8 in hepatic tissue were assayed by ELISA. All data were analyzed by one-way ANOVA, Pearson rank correlation or Spearman correlation. Results The contents of CXCL5 and CXCL8 were （0.8±0.7 ） ng/g and （6.2±3.7） ng/g in hepatic hemangioma group, （2.0±2.0） ng/g and （ 11.6±3.5 ） ng/g in liver fibrosis group and （ 17.1± 4.8 ） ng/g and （ 12.3±3.9） ng/g in liver cirrhosis group, with significant difference among the 3 groups （ F = 60. 050, 7. 690, P 〈 0.05）. The expression of CXCL5 was correlated with the content of alanine aminotransferase （ALT）, aspartate aminotransferase （AST） and prothrombin time （FT） （r = 0. 502, 0. 468, 0. 523, P 〈 0.05 ） ; the expression of CXCL8 was correlated with the content of ALT, AST, total bilirubin and PT （ r = 0. 477, 0. 504, 0. 537, 0. 431, P 〈 0.05 ）. Conclusions With the aggravation of hepatic fibrosis, the contents of CXCL5 and CXCL8 are increased with different patterns. The changes of CXCL5 and CXCL8 are related with the injury of liver, but the changes of CXCL5 and CXCL8 do not correspond with the degree of the injury of liver.