初探各实验条件对基于核磁共振的代谢组学研究结果的影响

Impact of Experimental Conditions on the Results of NMR-based Metabononmics Study

鉴于代谢组学样品的制备分析过程中,样品个体差异、环境因素和人为因素会对实验结果造成一定的影响,本研究对不同样本个体,不同实验操作人员的实验结果进行比较。通过这样的双向比较,排查出主要影响因素,为今后的实验奠定基础。实验选取多元统计中聚类分析的方法对12个样本进行衡量。实验结果表明,大鼠间差异即样本个体差异会对代谢组学研究结果产生一定的影响;而实验者不同即实验环境、人为因素对结果的影响不显著。也就是说,样本先天的个体差异相对与不同实验者所造成的环境差异在代谢组学中表现更为明显。

In the process of preparing and analyzing the metabonomics samples,there are lots of influential factors such as sample individual differences,environmental factors and human factors.Here we report a study on 3 healthy male SD rats of different samples carried out by different operators（n=4）.It was a two-way experiment designed to explore the impact factors and lay the foundation for future experiments.The cluster analysis was employed in comparing 12 samples.The results showed that the differences of rats between the sample groups had a certain influence on the metabonomics data,and there was relatively little variability between the 4 operators.That is to say,whilst the sample are rather different from one another,a given sample is generally quite similar to itself in metabonomics study.