人参皂甙Rg1对糖尿病肾病大鼠肾脏保护作用的机制研究

Effects of Ginsenoside Rg1 on Streptozocin-Induced Diabetic Nephropathy in Rats

观察人参皂甙Rg1对糖尿病大鼠肾脏的保护作用,采用链脲佐菌素(streptozotocin,STZ)腹腔注射建立糖尿病肾病(diabetic nephropathy,DN)大鼠模型,给药8周后处死各组大鼠,行肾功和24 h尿蛋白定量检查;高碘酸-希夫(氏)染色剂染色(periodic acid-Schiff,PAS)观察肾组织的病理变化;采用免疫散射比浊法检测血C-反应蛋白(cross reaction protein,CRP)水平;免疫放射法检测血肿瘤坏死因子-α(tumor necrosis factor,TNF-α)水平;免疫组织化学法检测单核/巨噬细胞表面特异性标志抗原(ectodermal dysplasia,ED-1)的表达;行酶联免疫吸附法(en-zyme-linked i mmunosorbent assay,ELISA)和实时荧光定量PCR(RT-PCR)检测肾组织转化生长因子-β1(trans-forming growth factor-β1,TGF-β1)水平。给予人参皂甙Rg1治疗的DN大鼠肾脏病理改变明显减轻,血肌酐、24 h尿蛋白、血CRP、TNF-α、ED-1、TGF-β1较模型组明显降低(P<0.05)。因此,人参皂甙Rg1能显著地减少TGF-β1和炎症反应因子水平,改善肾脏的病理损伤。

This study sought to assess the effect of Ginsenoside Rg1 on streptozocin-induced diabetic nephropathy in rats and to unveil the underlying mechanism.Diabetic nephropathy（DN） was induced by intraperitoneal injection of streptozocin（STZ）.Eight weeks after drug administration,the rats from each group were sacrificed.Serum creatine（Scr） and 24 hours urine protein,cross reaction protein（CRP） and tumor necrosis factor-α（TNF-α） were measured at the end of the study.The histological changes of renal interstitial tissues were observed by periodic acid-Schiff staining（PAS）.Immunohistochemical method was used to examine the expression levels of ectodermal dysplasia（ED-1）.The mRNA of transforming growth factor-β1（TGF-β1） was measured by real-time PCR（RT-PCR）,and the protein expression of TGF-β1 was surveyed by Enzyme-Linked Immunosorbent Assay（ELISA）.The renal pathological changes in DN rats given ginsenoside Rg1 treatment were ameliorated,and the expression levels of 24h urine protein,serum creatinine,CRP,TNF-α,ED-1 and TGF-β1 were significantly lower than those in the diabetic nephropathy group（P0.05）.So,we reach a conclusion that,in the experiment,Ginsenoside Rg1 obviously reduced TGF-β1 expression and the already-mentioned inflammatory reaction factors in the renal tissues and improved the renal pathological changes in DN rats.