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KiSS-1抑制骨肉瘤MG63细胞的侵袭和迁移 被引量：2

KiSS-1 inhibits invasion and migration of osteosarcoma MG63 cells
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摘要 目的:探讨人肿瘤转移抑制基因KiSS-1对人骨肉瘤MG63细胞侵袭、迁移能力的影响。方法:构建KiSS-1表达质粒pSNAV2.0-KiSS-1。pSNAV2.0-KiSS-1质粒转染骨肉瘤MG63细胞,经G418筛选稳定表达KiSS-1基因的MG63细胞。应用real-time PCR、Western blotting检测KiSS-1 mRNA和蛋白的表达。Transwell小室法检测MG63细胞的侵袭力,millicell小室、细胞划痕愈合实验检测KiSS-1基因对MG63细胞迁移能力的影响。结果:成功建立pSNAV2.0-KiSS-1质粒并稳定转染MG63细胞(MG63-KiSS-1细胞),MG63-KiSS-1细胞高表达KiSS-1 mRNA和蛋白。转染KiSS-1质粒后的MG63细胞侵袭力显著降低(P<0.05);millicell法、细胞划痕愈合实验证实转染KiSS-1质粒后的MG63细胞迁移力也明显降低(P<0.05)。结论:KiSS-1基因能显著抑制人骨肉瘤MG63细胞的侵袭和迁移能力,在骨肉瘤的转移中起重要作用。 Objective：To investigate the effects of tumor metastasis-suppressor gene KISS-1 on the invasion and migration abilities of osteosarcoma MG63 cells. Methods： KISS-1 expression plasmid pSNAV2.0-KISS-1 was constructed and transfected into MG63 cells. MG63 cells stably transfected with pSNAV2.0-KISS-1 （named MG63-KiSS-1 ） were selected by G418. KISS-1 mRNA and protein expression in MG63-KiSS-1 cells was examined by real-time PCR and Western blotting analysis, respectively. The invasion ability of MG63 cells was detected by transwell assay. The effects of KISS-1 on the invasion and migration abilities of MG63 cells were measured by millicell assay and cell scratch healing assay. Results： MG63 cells stably transfected with pSNAV2.0-KISS-1 were successfully established. MG63-KiSS-1/ cells highly expressed KISS-1 protein. The invasion ability of MG63 cells was significantly decreased after pSNAV2.0-KISS-1 transfection （P 〈 0.05）. The migration ability of MG63 cells was also significantly inhibited after pSNAV2.0-KISS-1 transfection as examined by millicell assay and cell scratch healing assay （ P 〈 0.05 ）. Conclusion： KISS-1 gene can significantly inhibit the invasion and migration abilities of osteosarcoma MG63 cells, which may play a key role in metastasis of osteosarcoma.
作者 沈荣凯 张俐 林建华
机构地区 福建医科大学附属第一医院骨科 福建中医学院骨伤科
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2010年第2期168-173,共6页 Chinese Journal of Cancer Biotherapy
基金 卫生部科学研究基金-福建卫生教育联合攻关计划(No.WKJ2008-2-042)~~
关键词 KiSS—1基因 骨肉瘤 MG63细胞 侵袭 迁移 KISS-1 gene osteosarcoma MG63 cell invasion migration
分类号 R714.1 [医药卫生—妇产科学]
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参考文献19

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二级参考文献6

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共引文献12

同被引文献16

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中国肿瘤生物治疗杂志
2010年 第2期

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