RhoGDI蛋白在肺癌组织中的表达及其与临床病理特征的关系

RhoGDI protein expression in lung cancer tissues and its relationship with clinicopathological characteristics

目的:探讨RhoGDI(Rhoguanine nucleotide dissociation inhibitor)蛋白在肺癌组织中的表达及其临床意义。方法:应用Western blotting检测苏州大学两所附属医院于2007-2008年收治的39例肺癌及5例肺炎性假瘤患者组织标本中RhoGDI蛋白的表达,并分析RhoGDI蛋白表达与患者临床病理指标的相互关系。结果:小细胞肺癌组织中RhoGDI蛋白的表达水平显著高于非小细胞肺癌和炎性假瘤[(0.903±0.228)vs(0.522±0.152),(0.485±0.095);均P<0.05)]。非小细胞肺癌组织中,低分化癌RhoGDI蛋白的表达水平显著高于中、高分化癌和炎性假瘤[(0.649±0.123)vs(0.458±0.138),(0.485±0.095);均P<0.05]。RhoGDI的表达在鳞癌与腺癌中没有显著差别。非小细胞肺癌Ⅰ～Ⅲ期的RhoGDI蛋白表达水平有逐渐增高趋势,但无显著性差别(F=0.6,P>0.05)。RhoGDI蛋白的表达与原发肿瘤大小(t=1.15,P>0.05)和淋巴结转移无明显相关(t=1.648,P>0.05)。非小细胞肺癌组织RhoGDI的表达水平与血清乳酸脱氢酶水平呈正相关(r=0.46,P<0.01)。结论:肺癌组织中RhoGDI蛋白表达的明显升高与肺癌的病理类型和分化程度有关。

Objective： To investigate the expression of Rho guanine nucleotide dissociation inhibitor（RhoGDI） protein in lung cancer and to evaluate its clinical significance. Methods： The expression of RhoGDI protein in 39 lung cancer specimens and 5 lung inflammatory pseudotumor tissues were detected by Western blotting analysis. The tissues were collected from patients treated in the two affiliated hospitals of Soochow University during 2007 -2008. The relationship between RhoGDI expression and the clinical and pathological characteristics of lung cancer were analyzed. Results： RhoGDI protein level in small-cell lung cancer（SCLC） tissues was significantly higher than those in non-small cell lung cancer （NSCLC） and inflammatory pseudotumor tissues （ [ 0. 903± 0. 228 ] vs [ 0. 522 ± 0. 152 ], [ 0. 485 ± 0. 095 ], P 〈 0. 05 ）. RhoGDI protein level was significantly higher in poorly-differentiated tumors than those in moderately-, highly-differentiated NSCLC and inflammatory pseudotumor tissues （ [0. 649 ± 0. 123 ] vs [0. 458 ± 0. 138 ], [0. 485±0. 095 ], P 〈0. 05 ）. RhoGDI protein level in squamous carcinoma had no difference with that in adenocarcinoma. The level of RhoGDI protein in NSCLC tissues was increased gradually when the pathology of tumor stage increased （ Ⅰ -Ⅲ ）, but with no significant difference was found among them （ F = 0.6, P 〉 0.05 ）. RhoGDI protein level in lung cancer was not related to the tumor size （ t = 1.15, P 〉 0.05 ） or lymphatic metastasis （ t = 1. 648, P 〉 0.05 ）. RhoGDI protein expression in NSCLC was positively correlated with the serum levels of lactate dehydrogenate （ r = 0. 46, P 〈 0.01 ）. Conclusion： RhoGDI protein expression is correlated with pathological characteristics and differentiation stages of lung cancer.