银杏叶提取物联合灯盏细辛对视网膜神经节细胞的保护作用

Protective effect of Ginaton and fleabane on retinal ganglion cells

目的:探讨银杏叶提取物联合灯盏细辛对兔慢性高眼压模型视网膜神经节细胞的保护作用。方法:采用α-糜蛋白酶(0.2mL)(2667.2μkat)注入家兔眼后房,造成实验性慢性高眼压。将造模成功的32只家兔随机分为4组,每组8只,分别给予以下治疗:银杏叶提取物150mg/kg(银杏叶组);灯盏细辛150mg/kg(灯盏细辛组);银杏叶提取物联合灯盏细辛皆灌胃,1次/d银杏叶提取加灯盏细辛(联合用药组);不做任何治疗(模型对照组)。4wk后各组分别进行视网膜神经节细胞凋亡检测正常视神经节细胞计数和光镜观察视网膜各层病理改变。结果:每组均可观察到阳性细胞,正常神经节细胞计数最多为联合用药组(889.00±40/mm2)、最少为模型对照组(581±24/mm2),P<0.01,光镜下模型对照组视网膜各层结构最紊乱,联合用药组视网膜各层组织最完整。结论:银杏叶提取物联合灯盏细辛能阻止高眼压导致视网膜神经节细胞发生凋亡。

AIM： To study the protective effect of Ginaton and fleabane on retinal ganglion cells in rabbit chronic high intraocular pressure model.METHODS： Chronic high intraocular pressure was created by injecting α-chymotrypsin（0.2mL）（2667.2μkat） into rabbit posterior chamber. Thirty-two rabbits of successful model were divided randomly into four groups, with 8 rabbits in each group, and then treated with different therapies as follows：intragastric administration with Ginaton 150mg/kg, once a day （Ginaton group）; intragastric administration with fleabane 150mg/kg, once a day （fleabane group）;intragastric administration with Ginaton and fleabane（combination therapy group）and control group. After 4 weeks of treatment, all groups were checked retinal ganglion cells apoptosis, normal ganglion cells counting ,pathological changes of retinal layers with light microscopy. RESULTS： Positive cells could be found in every group, the number of normal retinal ganglion cells was the largest in combination therapy group（889.00±40/mm2 ）, the least in control group（581±24/mm2 ）,P〈0.01. In light microscopy, the worst arrangement of retinal layers was in control group, and the best was in combination therapy group. CONCLUSION： Ginaton and fleabane could prevent high intraocular pressure caused apoptosis of retinal ganglion cells.