脂多糖干预后成牙本质细胞系MDPC-23中肿瘤坏死因子受体相关因子6基因表达的研究

Expression of TRAF6 gene in MDPC-23 cells as a response to LPS stimulus

目的观察不同浓度脂多糖(LPS)干预后,成牙本质细胞中肿瘤坏死因子受体相关因子6(TRAF6)基因表达的情况。方法体外培养小鼠永生化成牙本质细胞系MDPC-23,采用对照组不干预(即0μg/ml组)、10μg/ml和20μg/mlLPS干预MDPC-23后,通过反转录聚合酶链反应法(RT-PCR)和计算机图像分析方法,观察其TRAF6基因的表达变化。结果TRAF6在小鼠成牙本质细胞系MDPC-23中呈阳性表达,当LPS干预后,TRAF6基因表达呈现上调趋势。结论本实验表明TRAF6参与调控LPS引发的成牙本质细胞炎症损伤过程中的细胞因子网络,推测TRAF6可能影响龋病的形成和发展过程。

Objective To determine the expression of tumor necrosis factor receptor-associated factor-6（TRAF6） in MDPC-23 cell in response to different concentrations of LPS.Methods Mouse odontoblast-like cells（MDPC-23） were cultured in vitro.After MDPC-23 was intervened with 0 μg/ml（control group）,10 μg/ml and 20 μg/ml LPS,RT-PCR and computerized image analysis were applied to examine the changes of TRAF6 gene expression.Results Expression of TRAF6 gene tested positive in MDPC-23,which appeared up-regulated after LPS intervention.Conclusion TRAF6 might have a role in the development and progression of dental caries by involvement in cytokine network of LPS-induced MDPC-23 inflammatory injury.