P16蛋白在胃癌组织中的表达及临床意义

Study on the expression of P16 protein and its clinical significance in gastric carcinoma

目的:探讨p16基因在胃癌组织中的表达及在胃癌预后中的临床意义。方法:应用免疫组织化学链霉菌素-生物素法(streptaridin peroxidase,SP)对68例胃癌患者的胃癌组织和同期收集的25例正常胃黏膜行P16蛋白检测。结果:与正常胃黏膜组织中P16蛋白的表达率比较,胃癌组织中P16蛋白的阳性表达率明显降低(P<0.05);与高、中分化胃癌组织比较,低分化、未分化胃癌组织中P16蛋白的阳性表达率显著降低(P<0.05);在发生淋巴结转移的胃癌组织中,P16蛋白阳性表达率为28.57%,而未发生淋巴结转移的胃癌组织中,P16蛋白阳性表达率为61.53%,两组比较,差异有统计学意义(P<0.05);P16蛋白阳性表达与浸润深度之间无明显关系。结论:P16蛋白异常表达与胃癌的不良生物学行为有关,且检测P16蛋白的表达情况有助于判断胃癌的预后。

Objective： To explore the clinical significance of p16 gene expression in gastric carcinoma and its role in the prognosis of gastric carcinoma.Methods： The expression of P16 in 68 patients with gastric carcinoma were analyzed by immunohistochemical staining.Results： The expression levels of P16 protein in normal gastric tissues and gastric carcinoma tissues showed significant difference （P0.05）.The expression levels of P16 protein in differentiated types and undifferentiated types also showed significant difference （P0.05）.The positive rate of P16 protein in patients with lymphatic metastasis （28.57%） was significantly lower than that without lymphatic metastasis （61.53%） （P0.05）,and the positive rate of P16 protein was not related to the infiltration degrees of gastric carcinoma.Conclusion： The abnormal expression of P16 is related to the poor biological behavior and may be helpful to evaluate the prognosis of gastric carcinoma.