HPV感染和p53异常表达与宫颈鳞癌的关系

Relationship between the infection of HPV and expression of p53 in cervical squamous cell carcinoma

目的研究宫颈鳞癌与p53蛋白表达和HPV感染的关系,探讨宫颈鳞癌的形成机制。方法采用RT-PCR法分别检测p53基因在39例宫颈鳞癌组织和39例正常宫颈黏膜组织中的表达情况以及PCR方法检测HPV在这些组织中的感染情况。结果 HPV在宫颈鳞癌组中阳性率为48.72%(19/39),正常组织中为14.81%(4/27),p53基因表达在宫颈鳞癌组中阳性率为53.85%(21/39),正常组织中为18.52%(5/27),宫颈癌组的HPV感染和p53表达均高于正常组(P<0.05)。结论 HPV感染与p53基因的异常表达与宫颈鳞癌发生密切相关,联合检测能提高准确性。

Objective： To study the infection of HPV and expression of p53 in cervical squamous cell cancer and the correlation between them. Methods ： HPV DNA was tested by PCR, and the expression of p53 was examined by RT - PCR respectively in 39 cases of cervical squamous eell carcinoma and 27 cases of normal tissues. Results： In HPV - DNA analysis, 48. 72% （19/39） cases in cervical squamous cell cancer and 14. 81% （4/27） cases in normal tissues were positive. The expression of p53 was 53.85% （21/39） cases in cervical squamous cell cancer and 18. 52% （5/27） cases in normal tissues. The positive of HPV and p53 in cervical squamous cell cancer was more than that of normal tissues （ P 〈 0. 05 ）. Conclusion： The result suggested that HPV infection and p53 overexpression play a significant role in cervical squamous cell cancer, combined assay can improve the accuracy.